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Literature summary for 2.7.4.1 extracted from
- Production of aminoacyl prolines using the adenylation domain of nonribosomal peptide synthetase with class III polyphosphate kinase 2-mediated ATP regeneration (2018), J. Biosci. Bioeng., 125, 644-648 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | construction of an ATP regeneration system from AMP using PPK2, coupled with aminoacyl proline (Xaa-Pro) synthesis catalyzed by the adenylation domain of tyrocidine synthetase TycA-A. 0.87 mM of L-Trp-L-Pro is successfully synthesized from AMP after 72 h. Addition of inorganic diphosphatase increases the reaction rate by 14fold. When the coupling reaction is applied to whole-cell reactions in Escherichia coli, ATP is successfully regenerated from AMP, and 6.7 mM of L-Trp-L-Pro is produced after 24 h with the supplementation of 10 mM AMP. Also various other L-Xaa-L-Pro an be produced | Deinococcus proteolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus proteolyticus | F0RMA7 | - |
- |
| Deinococcus proteolyticus DSM 20540 | F0RMA7 | - |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Deipr_1912 | - |
Deinococcus proteolyticus |
| polyphosphate:nucleotide phosphotransferase | - |
Deinococcus proteolyticus |
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