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Literature summary for 2.7.4.1 extracted from
- Efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli (2020), J. Ind. Microbiol. Biotechnol., 47, 573-583 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli | Corynebacterium glutamicum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Corynebacterium glutamicum |  |
| Mg2+ | activates | Corynebacterium glutamicum | |
| Mn2+ | activates | Corynebacterium glutamicum | |
| Zn2+ | activates | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
SDS-PAGE | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | ANU33171.1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate(6) | i.e. hexametaphosphate | Corynebacterium glutamicum | ATP + phosphate(5) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPK | - |
Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Corynebacterium glutamicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: 83% of maximal activity, 50°C: 79% of maximal activity | Corynebacterium glutamicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
3 h, 10% loss of activity | Corynebacterium glutamicum |
| 40 | - |
3 h, 22% loss of activity | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Corynebacterium glutamicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
24 h, 20% loss of activity | Corynebacterium glutamicum |
| 9 | - |
24 h, 50% loss of activity | Corynebacterium glutamicum |
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Release 2023.1 (January 2023)
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