KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
ATP | pH 7.5, 30°C | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Sus scrofa | |
Mg2+ | divalent cation required, Mg2+ most efficient | Sus scrofa | |
Mn2+ | can partially replace Mg2+ in activation | Sus scrofa | |
Zn2+ | can partially replace Mg2+ in activation | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
1 * 21000, SDS-PAGE | Sus scrofa |
22000 | - |
- |
Sus scrofa |
22500 | - |
- |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Oxidation Stability | Organism |
---|---|
very unstable in absence of thiol compounds | Sus scrofa |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, BioGel P-150, hydroxylapatite, Blue Dextran-Sepharose | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate | ordered mechanism | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
69.1 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris-HCl, pH 7.5, 1 mM dithiothreitol, 50% glycerol, 15 months, 20% loss of acctivity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phosphomevalonate | (3R)-phosphomevalonate | Sus scrofa | ADP + 5-diphosphomevalonate | (3R)-diphosphomevalonate | r | |
ATP + 5-phosphomevalonate | absolute specificity for ATP | Sus scrofa | ADP + 5-diphosphomevalonate | (3R)-diphosphomevalonate | r | |
ATP + 5-phosphomevalonate | the equilibrium constant is near unity at pH 7.5 and 30°C | Sus scrofa | ADP + 5-diphosphomevalonate | (3R)-diphosphomevalonate | r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Sus scrofa |