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Literature summary for 2.7.4.2 extracted from
- The enzymes forming isopentenyl pyrophosphate from 5-phosphomevalonate (mevalonate 5-phosphate) in the latex of Hevea brasiliensis (1971), Biochem. J., 124, 407-417.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | slight stimulation at 10 mM | Hevea brasiliensis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
5-phosphomevalonate | pH 7.2, 30°C | Hevea brasiliensis | |
| 0.19 | - |
ATP | pH 7.2, 30°C | Hevea brasiliensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | can partially replace Mg2+ in activation | Hevea brasiliensis | |
| Fe2+ | maximal activation at a Fe2+/ATP ratio of 4:1 | Hevea brasiliensis | |
| Mg2+ | maximal activity at 5 mM MgCl2, maximal activity at a Mg2+/ATP ratio of 3:1, Mn2+ and Mg2+ most effective | Hevea brasiliensis | |
| Mn2+ | can partially replace Mg2+ in activation | Hevea brasiliensis | |
| Mn2+ | maximal activity at a Mn2+/ATP ratio of 1:1 | Hevea brasiliensis | |
| Mn2+ | Mn2+ and Mg2+ are the most effective | Hevea brasiliensis | |
| Zn2+ | can partially replace Mg2+ in activation | Hevea brasiliensis | |
| Zn2+ | maximal activation at a Zn2+/ATP ratio of 2:1 | Hevea brasiliensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hevea brasiliensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-200 | Hevea brasiliensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Hevea brasiliensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
- |
Hevea brasiliensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, 1 month, no loss of activity | Hevea brasiliensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phosphomevalonate | no activity with CTP and GTP | Hevea brasiliensis | ADP + 5-diphosphomevalonate | - |
? | |
| ITP + 5-phosphomevalonate | 6% of activity with ATP | Hevea brasiliensis | IDP + 5-diphosphomevalonate | - |
? | |
| UTP + 5-phosphomevalonate | 13% of activity with ATP | Hevea brasiliensis | UDP + 5-diphosphomevalonate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Hevea brasiliensis |
| 40 | - |
- |
Hevea brasiliensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
70% loss of activity after 30 min | Hevea brasiliensis |
| 45 | - |
complete loss of activity after 30 min | Hevea brasiliensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | approx. 50% of maximal activity at pH 6.5 and pH 8.2 respectively | Hevea brasiliensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | unstable below pH 6.0 and above pH 7.5 | Hevea brasiliensis |
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