Application | Comment | Organism |
---|---|---|
medicine | the enzymes of the mevalonate pathway of isopentenyl diphosphate biosynthesis represent potential for addressing a pressing human health concern, the development of antibiotics against resistent strains of the Gram-positive streptococci | Enterococcus faecalis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
ATP | - |
Enterococcus faecalis | |
0.19 | - |
(R,S)-5-phosphomevalonate | - |
Enterococcus faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM cannot replace Mn2+ or Mg2+ | Enterococcus faecalis | |
Mn2+ | preferred cation to activate the enzyme, optimal concentration about 4 mM | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence | Enterococcus faecalis |
48000 | - |
2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence | Enterococcus faecalis |
76000 | - |
analytical ultracentrifugation | Enterococcus faecalis |
81000 | - |
calculated from amino acid sequence | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | Enterococcus faecalis | mevalonate pathway of isopentenyl diphosphate biosynthesis | ADP + (R)-5-diphosphomevalonate | - |
? | |
ATP + (R)-5-phosphomevalonate | Enterococcus faecalis 41 | mevalonate pathway of isopentenyl diphosphate biosynthesis | ADP + (R)-5-diphosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Enterococcus faecalis 41 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.9 | - |
- |
Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | mevalonate pathway of isopentenyl diphosphate biosynthesis | Enterococcus faecalis | ADP + (R)-5-diphosphomevalonate | - |
? | |
ATP + (R)-5-phosphomevalonate | mevalonate pathway of isopentenyl diphosphate biosynthesis | Enterococcus faecalis 41 | ADP + (R)-5-diphosphomevalonate | - |
? | |
ATP + (R,S)-5-phosphomevalonate | - |
Enterococcus faecalis | ADP + (R,S)-5-diphosphomevalonate | - |
? | |
ATP + (R,S)-5-phosphomevalonate | - |
Enterococcus faecalis 41 | ADP + (R,S)-5-diphosphomevalonate | - |
? | |
CTP + (R,S)-5-phosphomevalonate | 0.4% activity | Enterococcus faecalis | CDP + (R,S)-5-diphosphomevalonate | - |
? | |
CTP + (R,S)-5-phosphomevalonate | 0.4% activity | Enterococcus faecalis 41 | CDP + (R,S)-5-diphosphomevalonate | - |
? | |
GTP + (R,S)-5-phosphomevalonate | 3.6% activity | Enterococcus faecalis | GDP + (R,S)-5-diphosphomevalonate | - |
? | |
GTP + (R,S)-5-phosphomevalonate | 3.6% activity | Enterococcus faecalis 41 | GDP + (R,S)-5-diphosphomevalonate | - |
? | |
TTP + (R,S)-5-phosphomevalonate | 1.6% activity | Enterococcus faecalis | TDP + (R,S)-5-diphosphomevalonate | - |
? | |
TTP + (R,S)-5-phosphomevalonate | 1.6% activity | Enterococcus faecalis 41 | TDP + (R,S)-5-diphosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
phosphomevalonate kinase | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Enterococcus faecalis |