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Literature summary for 2.7.4.2 extracted from

  • Chang, Q.; Yan, X.; Gu, S.; Liu, J.; Liang, D.
    Crystal structure of human phosphomevalonate kinase at 1.8 A resolution (2008), Proteins, 73, 254-258.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged enzyme, mixing of 0.002 ml of 10 mg/mL protein in 100 mM (NH4)2SO4, 20 mM HEPES, pH 7.6, with 0.002 ml reservoir solution containing 15% v/v pentaerythritol ethoxylate, 100 mM HEPES, pH 7.6, 15% v/v MPD, 15°C, 2-3 days, X-ray diffraction structure determination and analysis at 1.76 A resolution, SAD method Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-5-phosphomevalonate Homo sapiens
-
ADP + (R)-5-diphosphomevalonate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate
-
Homo sapiens ADP + (R)-5-diphosphomevalonate
-
?
ATP + (R)-5-phosphomevalonate PMK catalyzes the transfer of the gamma-phosphoryl group of ATP to (R)-5-phosphomevalonate, which results in the formation of ADP and (R)-5-diphosphomevalonate Homo sapiens ADP + (R)-5-diphosphomevalonate
-
?

Synonyms

Synonyms Comment Organism
More PMK is a member of the NMP superfamily Homo sapiens
PMK
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP binding site structure, overview Homo sapiens

General Information

General Information Comment Organism
metabolism phosphomevalonate kinase catalyzes the rate-limiting step for biosynthesis of isopentenyl diphosphate from mevalonate Homo sapiens