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Literature summary for 2.7.4.22 extracted from

  • Lee, S.E.; Kim, S.Y.; Kim, C.M.; Kim, M.K.; Kim, Y.R.; Jeong, K.; Ryu, H.J.; Lee, Y.S.; Chung, S.S.; Choy, H.E.; Rhee, J.H.
    The pyrH gene of Vibrio vulnificus is an essential in vivo survival factor (2007), Infect. Immun., 75, 2795-2801.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.22

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli, site directed mutagenesis Vibrio vulnificus

Protein Variants

Protein Variants Comment Organism
D77N no activity Vibrio vulnificus
R62H no activity Vibrio vulnificus
R62H/D77N strain CMM1474, reduced virulence Vibrio vulnificus

Organism

Organism UniProt Comment Textmining
Vibrio vulnificus
-
 and strain CMM1474
-
Vibrio vulnificus MO6-24/O
-
 and strain CMM1474
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant proteins Vibrio vulnificus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00014
-
 R62H/D77N mutant, without UTP, no activity with 1 mM UTP Vibrio vulnificus
0.0002
-
 R62H mutant, without UTP, no activity with 1 mM UTP Vibrio vulnificus
0.0003
-
 D77N mutant, without UTP, no activity with 1 mM UTP Vibrio vulnificus
0.00613
-
 wild-type, with 1 mM UTP Vibrio vulnificus
0.01238
-
 wild-type, without UTP Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
 Vibrio vulnificus ADP + UDP
-
 r
ATP + UMP
-
 Vibrio vulnificus MO6-24/O ADP + UDP
-
 r

Synonyms

Synonyms Comment Organism
pyrH
-
 Vibrio vulnificus
UMP kinase
-
 Vibrio vulnificus
UMPKs
-
 Vibrio vulnificus
uridine monophosphate kinase
-
 Vibrio vulnificus
uridylate kinase
-
 Vibrio vulnificus