Any feedback?
Literature summary for 2.7.4.24 extracted from
- Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress (2007), J. Biol. Chem., 282, 30763-30775.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activation of recombinant PPIP5K1 by hyperosmotic stress in HEK-293 cells | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Rattus norvegicus |
| functional overexpression of isozymes PPIP5K1 and PPIP5K2 in HEK-293 cells, expression of isozyme PPIP5K1 in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D332A | catalytically inactive mutant of isozyme PPIP5K1 | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 138000 | - |
about, isozyme PPIP5K2, mass spectrometry | Homo sapiens |
| 160000 | - |
about, isozyme PPIP5K1, mass spectrometry | Rattus norvegicus |
| 160000 | - |
about, isozyme PPIP5K1, mass spectrometry | Homo sapiens |
| 200000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
| 400000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate | Rattus norvegicus | (PP)2-InsP4 synthesis pathway, overview | ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate | - |
? |  |
| ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate | Homo sapiens | (PP)2-InsP4 synthesis pathway, overview | ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6PFW1 | - |
- |
| Rattus norvegicus | P0C644 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native PPIP5K1 from rat brain by anion exchange and heparin affinity chromatography, followed by nickel affinity chromatography and sucrose gradient centrifugation | Rattus norvegicus |
| recombinant FLAG-tagged or untagged isozymes PPIP5K1 and PPIP5K2 from HEK-293 cells and isozyme PPIP5K1 from Escherichia coli, respectively | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| brain | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate | (PP)2-InsP4 synthesis pathway, overview | Rattus norvegicus | ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate | (PP)2-InsP4 synthesis pathway, overview | Homo sapiens | ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | the enzyme contains a putative ATP-grasp kinase domain | Rattus norvegicus |
| More | the enzyme contains a putative ATP-grasp kinase domain | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diphosphoinositol pentakisphosphate kinase | - |
Rattus norvegicus |
| diphosphoinositol pentakisphosphate kinase | - |
Homo sapiens |
| PP-InsP5 kinase | - |
Rattus norvegicus |
| PP-InsP5 kinase | - |
Homo sapiens |
| PPIP5K | - |
Rattus norvegicus |
| PPIP5K | - |
Homo sapiens |
| PPIP5K1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Rattus norvegicus |
| 7.2 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
| ATP | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
