Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
CMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
0.165 | - |
dCMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
13.85 | - |
UMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
gel filtration | Mycobacterium tuberculosis |
23930 | - |
ESI-MS | Mycobacterium tuberculosis |
24060 | - |
calculated from amino acid sequence | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-80°C, no apparent loss of activity | Mycobacterium tuberculosis |
4°C, up to 30 days, recombinant CMK remains stable | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | the enzyme preferentially phosphorylates CMP and dCMP | Mycobacterium tuberculosis | ADP + CDP | - |
? | |
ATP + CMP | the enzyme preferentially phosphorylates CMP and dCMP | Mycobacterium tuberculosis H37Rv | ADP + CDP | - |
? | |
ATP + dCMP | the enzyme preferentially phosphorylates CMP and dCMP | Mycobacterium tuberculosis | ADP + dCDP | - |
? | |
ATP + dCMP | the enzyme preferentially phosphorylates CMP and dCMP | Mycobacterium tuberculosis H37Rv | ADP + dCDP | - |
? | |
ATP + UMP | UMP is a poor substrate | Mycobacterium tuberculosis | ADP + UDP | - |
? | |
ATP + UMP | UMP is a poor substrate | Mycobacterium tuberculosis H37Rv | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21000, gel filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CMK | - |
Mycobacterium tuberculosis |
CMP kinase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.2 | - |
UMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
30 | - |
dCMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
52 | - |
CMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
UMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
200 | - |
dCMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
1100 | - |
CMP | pH and temperature not specified in the publication | Mycobacterium tuberculosis |