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Literature summary for 2.7.4.26 extracted from

  • Vannice, J.C.; Skaff, D.A.; Keightley, A.; Addo, J.K.; Wyckoff, G.J.; Miziorko, H.M.
    Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl phosphate kinase as catalysts of the terminal enzyme reactions in an archaeal alternate mevalonate pathway (2014), J. Bacteriol., 196, 1055-1063.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.26

Cloned(Commentary)

Cloned (Comment) Organism
expression of an N-terminally His-tagged enzyme form in Haloferax volcanii H1209 Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 isopentenyl phosphate pH 7.5, 30°C Haloferax volcanii
0.29
-
 ATP pH 7.5, 30°C Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26417
-
 x * 26417, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + isopentenyl phosphate Haloferax volcanii the enzyme is involved in the mevalonate pathway in archaea ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate Haloferax volcanii DSM 3757 the enzyme is involved in the mevalonate pathway in archaea ADP + isopentenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GWT7
-
-
Haloferax volcanii DSM 3757 D4GWT7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + isopentenyl phosphate
-
 Haloferax volcanii ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate the enzyme is involved in the mevalonate pathway in archaea Haloferax volcanii ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Haloferax volcanii DSM 3757 ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate the enzyme is involved in the mevalonate pathway in archaea Haloferax volcanii DSM 3757 ADP + isopentenyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 26417, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Synonyms

Synonyms Comment Organism
HvIpk
-
 Haloferax volcanii
HVO_2762
-
 Haloferax volcanii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.3
-
 ATP pH 7.5, 30°C Haloferax volcanii
16.3
-
 isopentenyl phosphate pH 7.5, 30°C Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Haloferax volcanii

pI Value

Organism Comment pI Value Maximum pI Value
Haloferax volcanii calculated from sequence
-
 5.06