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Literature summary for 2.7.4.28 extracted from
- Cloning and characterization of Escherichia coli DUF299: A bifunctional ADP-dependent kinase - Pi-dependent pyrophosphorylase from bacteria (2010), BMC Biochem., 11, 000.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged protein expressed in Escherichia coli NM522 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
4 * 25000, gel filtration, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified protein stable for at least a week when stored on ice | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [phosphoenolpyruvate synthase]phosphate + phosphate | His and Thr residues phosphorylated (inactive) | Escherichia coli | phosphoenolpyruvate synthase + diphosphate | His residue phosphorylated (active) | ? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 25000, gel filtration, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DUF299 | - |
Escherichia coli |
| PEP synthetase regulatory protein | - |
Escherichia coli |
| PSRP | - |
Escherichia coli |
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