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Literature summary for 2.7.4.3 extracted from

  • Hamada, M.; Sumida, M.; Okuda, H.; Watanabe, T.; Nojima, M.; Kuby, S.A.
    Adenosine triphosphate-adenosine-5-monophosphate phosphotransferase from normal human liver mitochondria. Isolation, chemical properties, and immunochemical comparison with Duchenne dystrophic serum aberrant adenylate kinase (1982), J. Biol. Chem., 257, 13120-13128.
    View publication on PubMed
Search for more literature for 2.7.4.3

Crystallization (Commentary)

Crystallization (Comment) Organism
liver Homo sapiens
muscle Homo sapiens

General Stability

General Stability Organism
stable to dialysis in the presence of 4 mM dithioerythritol Homo sapiens
Stable to freeze-thawing Homo sapiens
unstable in dilute solution, 0.2 mg/ml, dithioerythritol, and bovine serum albumin stabilize Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25200
-
 liver, sedimentation equilibrium Homo sapiens
26349
-
 1 * 26349, calculated from amino acid analysis Homo sapiens
26350
-
 calculated from amino acid analysis Homo sapiens
26500
-
 1 * 26500, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 post mortem
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
280
-
 30°C, pH 7.4 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, several weeks Homo sapiens
3°C, 5-10% loss within 1 month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-(3-thio)triphosphate + AMP muscle: reaction at 97% the rate of ATP, liver mitochondria: reaction at 70% the rate of ATP Homo sapiens adenosine 5'-diphosphate + adenosine 5'-(3-thio)diphosphate
-
 ?
ADP + ADP no substrates: adenosine 5'-(2-thio)diphosphate, adenosine diphosphate 3'-diphosphate Homo sapiens ATP + AMP
-
 ?
ATP + adenosine 5'-thiophosphate muscle: reaction at 56% the rate of AMP, liver mitochondria: reaction at 95% the rate of AMP Homo sapiens ?
-
 ?
ATP + AMP no substrates are adenosine triphosphate 3'-diphosphate, adenosine-5'-(3-thio)triphosphate/adenosine 5'-thiophosphate Homo sapiens ADP + ADP
-
 r
ATP + AMP no substrates of the reverse reaction: adenosine 5'-(2-thio)diphosphate, adenosine diphosphate 3'-diphosphate Homo sapiens ADP + ADP
-
 r
ATP + AMP-3'-diphosphate muscle: reaction at 57% the rate of AMP, liver mitochondria: reaction at 86% the rate of AMP Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26500, SDS-PAGE Homo sapiens
monomer 1 * 26349, calculated from amino acid analysis Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 AMP + ATP Homo sapiens
7.9
-
 ADP + ADP Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.2 about half-maximal activity at pH 6.5 and 8.2 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 1 h, at 0°C, quite stable Homo sapiens
11.5
-
 1 h, at 0°C, quite stable Homo sapiens