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Literature summary for 2.7.4.3 extracted from

  • Ito, Y.; Tomasselli, A.G.; Noda, L.H.
    ATP:AMP phosphotransferase from bakers yeast. Purification and properties (1980), Eur. J. Biochem., 105, 85-92.
    View publication on PubMed
Search for more literature for 2.7.4.3

Activating Compound

Activating Compound Comment Organism Structure
NaCl 0.5 M Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Saccharomyces cerevisiae
Mg2+ at high concentrations; required for enzyme activity at low concentrations Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ not Saccharomyces cerevisiae
Ca2+ binding of substrates also takes place in the absence of metal ions Saccharomyces cerevisiae
Ca2+ in decreasing order of efficiency: Mg2+ and Ca2+, equally efficient, Co2+, Mn2+, Ni2+ Saccharomyces cerevisiae
Ca2+ requirement, as good as Mg2+ Saccharomyces cerevisiae
Co2+ requirement Saccharomyces cerevisiae
Fe2+ not Saccharomyces cerevisiae
Mg2+ requirement Saccharomyces cerevisiae
Mg2+ binding of substrates also takes place in the absence of metal ions Saccharomyces cerevisiae
Mg2+ in decreasing order of efficiency: Mg2+ and Ca2+, equally efficient, Co2+, Mn2+, Ni2+ Saccharomyces cerevisiae
Mg2+ inhibits at high concentrations Saccharomyces cerevisiae
Mn2+ binding of substrates also takes place in the absence of metal ions Saccharomyces cerevisiae
Mn2+ Mg2+ and Ca2+, equally efficient, Co2+, Mn2+, Ni2+ Saccharomyces cerevisiae
additional information no activation by Sr2+ Saccharomyces cerevisiae
Zn2+ not Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
4°C, pH 6-9, 2 weeks Saccharomyces cerevisiae
deep frozen, pH 6-9, 20 mg enzyme/ml, 4 months, but 60% loss of activity within a few days at pH 5 and 10 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP best substrates Saccharomyces cerevisiae ADP + ADP
-
 ?
ATP + AMP no substrates are ATP/IMP Saccharomyces cerevisiae ADP + ADP
-
 ?
ATP + AMP no substrates are ATP/UMP Saccharomyces cerevisiae ADP + ADP
-
 ?
ATP + AMP no substrates are ATP/GMP Saccharomyces cerevisiae ADP + ADP
-
 ?
ATP + CMP
-
 Saccharomyces cerevisiae ADP + ?
-
 ?
ATP + dAMP reaction at 11% the rate of AMP Saccharomyces cerevisiae ADP + dADP
-
 ?
CTP + AMP reaction at 13% the rate of ATP Saccharomyces cerevisiae ADP + CDP
-
 ?
dATP + AMP at the same rate as ATP Saccharomyces cerevisiae dADP + ADP
-
 ?
GTP + AMP reaction at 13% the rate of AMP Saccharomyces cerevisiae ADP + GDP
-
 ?
ITP + AMP 9% the rate of ATP Saccharomyces cerevisiae IDP + ADP
-
 ?
ITP + AMP not ATP/IMP Saccharomyces cerevisiae IDP + ADP
-
 ?
UTP + AMP reaction at 11% the rate of AMP Saccharomyces cerevisiae ADP + UDP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27500, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 20 h stable, pH 6-9 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 11 about half-maximal activity at pH 6 and 11, ADP + ADP Saccharomyces cerevisiae
6 10 about half-maximal activity at pH 6 and 10, AMP + ATP Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae isoelectric focusing
-
 5.7