Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Geobacillus stearothermophilus | |
AMP | - |
Escherichia coli | |
AMP | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | 0.037 | ATP | ADP, 37°C | Geobacillus stearothermophilus | |
0.038 | 0.04 | AMP | cosubstrate ATP, 30°C | Escherichia coli | |
0.048 | 0.051 | ATP | cosubstrate AMP, 30°C | Escherichia coli | |
0.076 | 0.083 | AMP | 37°C | Geobacillus stearothermophilus | |
0.09 | 0.092 | ADP3- | ADP, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | not | Escherichia coli | |
Zn2+ | requirement, tightly bound, 0.8 mol Zn2+ per mol protein, atomic absorption spectrophotometry | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24135 | - |
1 * 24135, calculated from nucleotide sequence | Geobacillus stearothermophilus |
24140 | - |
calculated from nucleotide sequence | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2400 | - |
- |
Escherichia coli |
3000 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + ADP | - |
Escherichia coli | ATP + AMP | - |
r | |
ADP + ADP | - |
Geobacillus stearothermophilus | ATP + AMP | - |
r | |
ATP + AMP | - |
Escherichia coli | ADP + ADP | - |
r | |
ATP + AMP | - |
Geobacillus stearothermophilus | ADP + ADP | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
monomer | 1 * 24135, calculated from nucleotide sequence | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
65 | - |
- |
Geobacillus stearothermophilus |