Protein Variants | Comment | Organism |
---|---|---|
S1202R | mutation of RAD50 signature motif, 10-50% of wild-type activity in formation of ADP, formation of ATP is not affected | Homo sapiens |
S1205R | mutation of RAD50 signature motif, 5-10% of wild-type activity in formation of ADP, formation of ATP is not affected. Complete loss of spore viability in the wild-type/S1205R heterozygote | Saccharomyces cerevisiae |
S1205R | mutation in Rad50 protein, mutant protein is expressed in yeast and forms a complete complex with Mre11. Mutant does not support spore viability. telomeres are as severely shortened as in a rad50 deletion strain | Saccharomyces cerevisiae |
S793R | mutation in Rad50 singature motif. Mutant shows lower levels of both ATPase and adenylate kinase activity relative to the wild-type enzyme, consistent with an overall deficiency in ATP binding | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-pentaphosphate | inhibits the RAD50 phosphoryl transfer reaction but not ATP hydrolysis | Homo sapiens | |
adenosine 5'-pentaphosphate | inhibits the RAD50 phosphoryl transfer reaction but not ATP hydrolysis | Saccharomyces cerevisiae | |
P1,P5-di(adenosine-5')pentaphosphate | inhibitory to Rad50 phosphoryl transfer reaction but not to ATP hydrolysis | Homo sapiens | |
P1,P5-di(adenosine-5')pentaphosphate | completely abolishes adenylate kinase activity but does not affect ATPase activity | Pyrococcus furiosus | |
P1,P5-di(adenosine-5')pentaphosphate | inhibitory to Rad50 phosphoryl transfer reaction but not to ATP hydrolysis. Inhibitor blocks DNA tethering in vitro and in cell-free extracts | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Xenopus laevis | adenylate kinase activity Is required for Mre11/Rad50-mediated DNA tethering | ? | - |
? | |
additional information | Homo sapiens | adenylate kinase activity of the Mre11/Rad50 complex, which is part of a DNA repair complex, promotes DNA-DNA associations | ? | - |
? | |
additional information | Saccharomyces cerevisiae | Rad50 adenylate kinase activity is required for DNA tethering | ? | - |
? | |
additional information | Pyrococcus furiosus | Rad50 adenylate kinase activity is required for DNA tethering | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
DNA repair complex components Mre11 and Rad50 show adenylate kinase activity when in complex Mre11/Rad50 | - |
Homo sapiens | - |
Mre11/Rad50 complex, part of a DNA repair complex | - |
Pyrococcus furiosus | - |
Mre11/Rad50 complex, part of a DNA repair complex | - |
Saccharomyces cerevisiae | - |
DNA repair complex components Mre11 and Rad50 show adenylate kinase activity when in complex Mre11/Rad50 | - |
Saccharomyces cerevisiae | - |
Mre11/Rad50 complex, part of a DNA repair complex | - |
Xenopus laevis | - |
DNA repair complex components Mre11 and Rad50 show adenylate kinase activity when in complex Mre11/Rad50 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP | - |
Homo sapiens | ATP + AMP | - |
r | |
ADP | - |
Saccharomyces cerevisiae | ATP + AMP | - |
? | |
ADP | - |
Xenopus laevis | ATP + AMP | - |
r | |
ADP | - |
Homo sapiens | AMP + ATP | - |
r | |
ADP | - |
Saccharomyces cerevisiae | AMP + ATP | - |
r | |
ADP | - |
Pyrococcus furiosus | AMP + ATP | - |
r | |
ATP + AMP | - |
Homo sapiens | 2 ADP | - |
r | |
ATP + AMP | - |
Saccharomyces cerevisiae | 2 ADP | - |
? | |
ATP + AMP | - |
Xenopus laevis | 2 ADP | - |
r | |
additional information | adenylate kinase activity Is required for Mre11/Rad50-mediated DNA tethering | Xenopus laevis | ? | - |
? | |
additional information | adenylate kinase activity of the Mre11/Rad50 complex, which is part of a DNA repair complex, promotes DNA-DNA associations | Homo sapiens | ? | - |
? | |
additional information | Rad50 adenylate kinase activity is required for DNA tethering | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Rad50 adenylate kinase activity is required for DNA tethering | Pyrococcus furiosus | ? | - |
? |