Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta cells | Jeotgalibacillus marinus |
Crystallization (Comment) | Organism |
---|---|
with the inhibitor P1,P5-di(adenosine-5')-pentaphosphate bound to the active site, sitting drop vapor diffusion method, using 1.5 M sodium citrate pH 6.5, 150 mM sodium chloride, 0.5% n-dodecyl-N,N-dimethylamine-N-oxide, at 10°C | Jeotgalibacillus marinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
P1,P5-di(adenosine-5')pentaphosphate | - |
Jeotgalibacillus marinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
ATP | at 30°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 6 5mM KCl | Jeotgalibacillus marinus | |
0.002 | - |
ATP | at 22°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl | Jeotgalibacillus marinus | |
0.002 | - |
ATP | at 40°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl | Jeotgalibacillus marinus | |
0.0214 | - |
AMP | at 22°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl | Jeotgalibacillus marinus | |
0.0216 | - |
AMP | at 40°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl | Jeotgalibacillus marinus | |
0.024 | - |
AMP | at 30°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl | Jeotgalibacillus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Jeotgalibacillus marinus | Q6B341 | strain ATCC 29841 | - |
Purification (Comment) | Organism |
---|---|
Q-XL Sepharose column chromatography, Affi-Gel Blue resin column chromatography, and Superdex-200 gel filtration | Jeotgalibacillus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | - |
Jeotgalibacillus marinus | ADP + ADP | - |
? |