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Literature summary for 2.7.4.3 extracted from
- Crystal structure of human adenylate kinase 4 (L171P) suggests the role of hinge region in protein domain motion (2009), Biochem. Biophys. Res. Commun., 379, 92-97.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant enzyme L171P, hanging drop vapor diffusion method, 4°C under conditions of 1.22-1.28 M (NH4)2SO4 and 0.1 M Tris-HCl, pH 8.5 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L171P | the mutation dramatically changes the orientation of the LID domain, which can be described as a twisted and closed conformation in contrast to the open and closed conformations in other adenylate kinases | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P27144 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose column chromatography and Superdex 75 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Homo sapiens | ADP + ADP | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenylate kinase 4 | - |
Homo sapiens |
| AK4 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | adenylate kinase 4 is a unique member of the adenylate kinases family which shows no enzymatic activity in vitro | Homo sapiens |
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Release 2023.1 (January 2023)
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