Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga neapolitana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
P1,P5-di(adenosine-5') pentaphosphate | - |
Escherichia coli | |
P1,P5-di(adenosine-5') pentaphosphate | - |
Thermotoga neapolitana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Escherichia coli | |
Mg2+ | required for activity | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Thermotoga neapolitana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation and Q-Sepharose column chromatography | Escherichia coli |
ammonium sulfate fractionation and Q-Sepharose column chromatography | Thermotoga neapolitana |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | - |
Escherichia coli | ADP + ADP | - |
r | |
ATP + AMP | - |
Thermotoga neapolitana | ADP + ADP | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
highly active at 30°C | Escherichia coli |
30 | - |
highly active at 30°C | Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
the melting temperature of the free enzyme is at 52°C | Escherichia coli |
99 | - |
the melting temperature of the free enzyme is at 99°C | Thermotoga neapolitana |