General Stability | Organism |
---|---|
enzyme is fully active in air | Methanococcus voltae |
enzyme is fully active in air | Methanotorris igneus |
enzyme is fully active in air | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 1 mM, complete inhibition of ADP-dependent ATP production | Methanococcus voltae | |
P1,P5-di(adenosine-5')pentaphosphate | - |
Methanocaldococcus jannaschii | |
P1,P5-di(adenosine-5')pentaphosphate | 50% inhibition at 50 mM, whether assayed in the direction of ATP formation from ADP or of ATP conversion to ADP | Methanococcus voltae | |
P1,P5-di(adenosine-5')pentaphosphate | - |
Methanothermococcus thermolithotrophicus | |
P1,P5-di(adenosine-5')pentaphosphate | - |
Methanotorris igneus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Methanothermococcus thermolithotrophicus | 5829 | - |
cytosol | - |
Methanococcus voltae | 5829 | - |
cytosol | - |
Methanotorris igneus | 5829 | - |
cytosol | - |
Methanocaldococcus jannaschii | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | maximum stimulation at 100 mM | Methanococcus voltae | |
K+ | maximum stimulation at 100 mM | Methanocaldococcus jannaschii | |
K+ | maximum stimulation at 200 mM | Methanotorris igneus | |
K+ | maximum stimulation at 400 mM | Methanothermococcus thermolithotrophicus | |
Mg2+ | activity is Mg2+ dependent | Methanococcus voltae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Methanococcus voltae |
25000 | - |
x * 25000 (approximately), SDS-PAGE | Methanothermococcus thermolithotrophicus |
25000 | - |
x * 25000 (approximately), SDS-PAGE | Methanotorris igneus |
25000 | - |
x * 25000 (approximately), SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | P43409 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | P43409 | - |
- |
Methanococcus voltae | P43411 | - |
- |
Methanothermococcus thermolithotrophicus | P43410 | - |
- |
Methanotorris igneus | P43408 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermococcus thermolithotrophicus |
- |
Methanococcus voltae |
- |
Methanotorris igneus |
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanococcus voltae |
56 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanotorris igneus |
81 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanothermococcus thermolithotrophicus |
89 | - |
substrate: ATP, pH and temperature not specified in the publication | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | - |
Methanothermococcus thermolithotrophicus | 2 ADP | - |
r | |
ATP + AMP | - |
Methanococcus voltae | 2 ADP | - |
r | |
ATP + AMP | - |
Methanotorris igneus | 2 ADP | - |
r | |
ATP + AMP | - |
Methanocaldococcus jannaschii | 2 ADP | - |
r | |
ATP + AMP | - |
Methanocaldococcus jannaschii DSM 2661 | 2 ADP | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Methanococcus voltae |
? | x * 25000 (approximately), SDS-PAGE | Methanothermococcus thermolithotrophicus |
? | x * 25000 (approximately), SDS-PAGE | Methanotorris igneus |
? | x * 25000 (approximately), SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Methanococcus voltae |
70 | - |
- |
Methanothermococcus thermolithotrophicus |
70 | - |
- |
Methanotorris igneus |
80 | 90 | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, the enzyme maintains 77.5% residual activity compared with the enzyme stored on ice | Methanococcus voltae |
59 | - |
1 h, the enzyme maintains 47% residual activity compared with the enzyme stored on ice | Methanococcus voltae |
68 | - |
t1/2: 30 min | Methanocaldococcus jannaschii |
68 | - |
t1/2: 4 min | Methanococcus voltae |
68 | - |
t1/2: 25 min | Methanothermococcus thermolithotrophicus |
68 | - |
t1/2: 43 min | Methanotorris igneus |
89 | - |
t1/2: 1.5 min | Methanococcus voltae |
89 | - |
t1/2: 17 min | Methanotorris igneus |
89 | - |
t1/2: 6.0 min | Methanothermococcus thermolithotrophicus |
89 | - |
t1/2: 6.0 min | Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | enzyme shows similar activity over a pH range from 6 to 10 | Methanococcus voltae |