Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | at higher concentrations, substrate inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | 0.1 | GDP | cosubstrate ATP, NDP-kinase I and II, 37°C, pH 7.4 | Spinacia oleracea | |
0.89 | - |
ATP | cosubstrate GDP, NDP-kinase II, 37°C, pH 7.4 | Spinacia oleracea | |
2 | - |
ATP | NDP-kinase I, 37°C, pH 7.4 | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Spinacia oleracea |
16000 | - |
6 * 16000, isozyme I, SDS-PAGE | Spinacia oleracea |
18000 | - |
6 * 18000, SDS-PAGE, isozyme II | Spinacia oleracea |
92000 | - |
isozyme I, gel filtration | Spinacia oleracea |
110000 | - |
isozyme II, gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 isozymes: NDP-kinase I and II | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | the catalytic mechanism involves a phosphoenzyme intermediate | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1250 | - |
NDP-kinase I, 37°C, pH 7.4 | Spinacia oleracea |
2160 | - |
NDP-kinase II, 37°C, pH 7.4 | Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20°C, 0.05 M 2-(N-morpholino)ethanesulfonic acid, pH 5.5, 1 mM EDTA, 0.2 mM PMSF, 0.1 M NaCl, 5 mM 2-mercaptoethanol, 0.1% Lubrol, several months | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-S-ATP + GDP | poor substrate | Spinacia oleracea | ADP + gamma-S-GTP | - |
? | |
gamma-S-GTP + GDP | poor substrate | Spinacia oleracea | GDP + gamma-S-GTP | - |
? | |
GTP + ADP | - |
Spinacia oleracea | GDP + ATP | - |
r | |
NTP + nucleoside diphosphate | - |
Spinacia oleracea | NDP + NTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 16000, isozyme I, SDS-PAGE | Spinacia oleracea |
hexamer | 6 * 18000, SDS-PAGE, isozyme II | Spinacia oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Spinacia oleracea |
37 | - |
assay at | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Spinacia oleracea |