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Literature summary for 2.7.4.6 extracted from

  • Mocan, I.; Georgescauld, F.; Gonin, P.; Thoraval, D.; Cervoni, L.; Giartosio, A.; Dabernat-Arnaud, S.; Crouzet, M.; Lacombe, M.; Lascu, I.
    Protein phosphorylation corrects the folding defect of the neuroblastoma (S120G) mutant of human nucleoside diphosphate kinase A/Nm23-H1 (2007), Biochem. J., 403, 149-156.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes Homo sapiens

Protein Variants

Protein Variants Comment Organism
H118N/S120G site-directed mutagenesis, the mutant is inactive and expressed as insoluble protein in inclusion bodies Homo sapiens
S120G naturally mutation occuring in neuroblastoma, the mutant has a folding defect, the urea-denatured protein cannot refold in vitro Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgATP promotes correct folding and association Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NDP Homo sapiens MgATP promotes correct folding and association ADP + NTP
-
?
additional information Homo sapiens NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P15531
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the molten globule folding intermediate can bind ATP and be autophosphorylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes by anion exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuroblastoma cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NDP
-
Homo sapiens ADP + NTP
-
?
ATP + NDP MgATP promotes correct folding and association Homo sapiens ADP + NTP
-
?
additional information NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
hexamer MgATP promotes correct folding and association, but MgATP does not shift the equilibrium between the folding intermediate and the native monomer in the presence of 2-3 M urea. The molten globule folding intermediate can bind ATP and be autophosphorylated Homo sapiens

Synonyms

Synonyms Comment Organism
NDP kinase A
-
Homo sapiens
NDPKA
-
Homo sapiens
Nm23-H1
-
Homo sapiens
nucleoside diphosphate kinase A
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens