Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H118N/S120G | site-directed mutagenesis, the mutant is inactive and expressed as insoluble protein in inclusion bodies | Homo sapiens |
S120G | naturally mutation occuring in neuroblastoma, the mutant has a folding defect, the urea-denatured protein cannot refold in vitro | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgATP promotes correct folding and association | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | Homo sapiens | MgATP promotes correct folding and association | ADP + NTP | - |
? | |
additional information | Homo sapiens | NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15531 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the molten globule folding intermediate can bind ATP and be autophosphorylated | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes by anion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuroblastoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | - |
Homo sapiens | ADP + NTP | - |
? | |
ATP + NDP | MgATP promotes correct folding and association | Homo sapiens | ADP + NTP | - |
? | |
additional information | NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | MgATP promotes correct folding and association, but MgATP does not shift the equilibrium between the folding intermediate and the native monomer in the presence of 2-3 M urea. The molten globule folding intermediate can bind ATP and be autophosphorylated | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NDP kinase A | - |
Homo sapiens |
NDPKA | - |
Homo sapiens |
Nm23-H1 | - |
Homo sapiens |
nucleoside diphosphate kinase A | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |