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Literature summary for 2.7.4.6 extracted from
- Investigation of chimerical and tagged forms of recombinant rat nucleoside diphosphate kinases alpha and beta. Interaction with rhodopsin-transducin complex and thermal stability (2007), Biochemistry (Moscow), 72, 835-842.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of HA-tagged chimeric mutants NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152 | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of chimeric mutants of NDP kinase alpha and NDP kinase beta, i.e. NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152 | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the acid-induced binding of NDP kinase HA-alpha1-130beta131-152 to ROS photoreceptor membranes is prevented by addition of NaCl or KCl | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of membrane binding of recombinat wild-type isozymes and chimeric mutants NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152 to N-membranes | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
6 * 17000, about | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GDP | Rattus norvegicus | an essential function of NDP kinase in ROS is phosphorylation of GDP to GTP, which is crucial for providing the functional cycle of transducin independently of its activation | ADP + GTP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
isozymes NDP kinase alpha and NDP kinase beta | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant HA-tagged chimeric mutants NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152 by one-step affinity chromatography using an ATP-resin | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GDP | - |
Rattus norvegicus | ADP + GTP | - |
? | |
| ATP + GDP | an essential function of NDP kinase in ROS is phosphorylation of GDP to GTP, which is crucial for providing the functional cycle of transducin independently of its activation | Rattus norvegicus | ADP + GTP | - |
? | |
| additional information | interaction of recombinant NDP kinases alpha and beta and their mutant chimeric derivatives with rhodopsin-transducin complex, binding to ROS photoreceptor membranes, overview | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 17000, about | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDP kinase alpha | - |
Rattus norvegicus |
| NDP kinase beta | - |
Rattus norvegicus |
| nucleoside diphosphate kinases alpha | - |
Rattus norvegicus |
| nucleoside diphosphate kinases beta | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability of recombinant NDP kinases alpha and beta and their mutant chimeric derivatives, NDP kinase beta, NDP kinase beta1-130alpha131-152, NDP kinase HA-beta1-130alpha131-152, and NDP kinase HA-beta are inactivated at a lower temperature with T1/2 of 51-54°C, overview | Rattus norvegicus |
| 50 | - |
T1/2 of isozyme NDP kinase beta | Rattus norvegicus |
| 61.5 | - |
T1/2 of isozyme NDP kinase alpha | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.1 | assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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