Cloned (Comment) | Organism |
---|---|
gene ndk, overexpressionin strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapor diffusion method, mixing 200 nL of protein solution, containing 15 mg/mL protein, with 200 nL of reservoir solution containing 0.2 M ammonium sulphate, 0.1 M sodium acetate/acetic acid buffer, pH 4.6, with either 30% PEG 2000 monomethyl ether or 25% PEG 4000, 20°C, a few hours, X-ray diffraction structure determination and analysis at 1.62 A resolution | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene ndk | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain BL21(DE3) by anion exchange chromatography, ammonium sulfate fractionation, dialysis, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NDPK catalyzes the transfer of gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the enzyme shows a tetrameric quaternary structure, the relative orientation of interacting dimers facing either the convex or the concave side of their central sheet, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
NDPK | - |
Escherichia coli |
nucleoside diphosphate kinase | - |
Escherichia coli |