Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.6 extracted from

  • Yamamura, A.; Ichimura, T.; Kamekura, M.; Mizuki, T.; Usami, R.; Makino, T.; Ohtsuka, J.; Miyazono, K.; Okai, M.; Nagata, K.; Tanokura, M.
    Molecular mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinases (2009), Biophys. J., 96, 4692-4700.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.6

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer (pH 7.6), 18% (w/v) PEG 400, and 10 mM EDTA, at 20°C Haloarcula sinaiiensis
sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer (pH 7.6), 18% (w/v) PEG 400, and 10 mM EDTA, at 20°C Haloarcula quadrata

Protein Variants

Protein Variants Comment Organism
R31C the mutation destabilizes the hexameric assembly, and causes dissociation to less active dimers at low salt concentrations Haloarcula quadrata

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ optimal NaCl concentration is 1 M Haloarcula quadrata
Na+ optimal NaCl concentration is 2 M Haloarcula sinaiiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 in the presence of 0.5 M NaCl, sedimentation velocity Haloarcula sinaiiensis
104000
-
 in the presence of 2 M NaCl, sedimentation velocity Haloarcula quadrata
111000
-
 in the presence of 2 M NaCl, sedimentation velocity Haloarcula sinaiiensis
114000
-
 in the presence of 0.5 M NaCl, sedimentation velocity Haloarcula quadrata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NDP Haloarcula sinaiiensis
-
 ADP + NTP
-
 ?
ATP + NDP Haloarcula quadrata
-
 ADP + NTP
-
 ?

Organism

Organism UniProt Comment Textmining
Haloarcula quadrata
-
-
-
Haloarcula sinaiiensis Q401C6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Haloarcula sinaiiensis
Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Haloarcula quadrata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + GTP
-
 Haloarcula sinaiiensis ATP + GDP
-
 ?
ADP + GTP
-
 Haloarcula quadrata ATP + GDP
-
 ?
ATP + NDP
-
 Haloarcula sinaiiensis ADP + NTP
-
 ?
ATP + NDP
-
 Haloarcula quadrata ADP + NTP
-
 ?

Subunits

Subunits Comment Organism
hexamer present as a hexamer under a wide range of salt concentrations (0.2-4 M NaCl) Haloarcula sinaiiensis
hexamer present as a hexamer under a wide range of salt concentrations (0.2-4 M NaCl) Haloarcula quadrata

Synonyms

Synonyms Comment Organism
NDK
-
 Haloarcula sinaiiensis
NDK
-
 Haloarcula quadrata
nucleoside diphosphate kinase
-
 Haloarcula sinaiiensis
nucleoside diphosphate kinase
-
 Haloarcula quadrata

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Haloarcula sinaiiensis
ATP
-
 Haloarcula quadrata