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Literature summary for 2.7.4.6 extracted from
- NaCl-activated nucleoside diphosphate kinase from extremely halophilic archaeon, Halobacterium salinarum, maintains native conformation without salt (2001), FEBS Lett., 493, 134-138.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Halobacterium salinarum |
General Stability
| General Stability | Organism |
|---|---|
| nucleoside diphosphate kinase prepared from Halobacterium salinarum is active and stable in the absence of salt. Recombinant nucleoside diphosphate kinase expressed in Escherichia coli requires salt for activation in vitro, but once it acquires the proper folding, it no longer requires the presence of salts for its activity and stability | Halobacterium salinarum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | nucleoside diphosphate kinase prepared from Halobacterium salinarum is active and stable in the absence of salt. Recombinant nucleoside diphosphate kinase expressed in Escherichia coli requires salt for activation in vitro, but once it acquires the proper folding, it no longer requires the presence of salts for its activity and stability | Halobacterium salinarum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Halobacterium salinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Halobacterium salinarum |
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Release 2023.1 (January 2023)
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