Cloned (Comment) | Organism |
---|---|
gene ndk, recombinant expression in Escherichia coli strain BL21Star(DE3) | Pseudoalteromonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
D135A/E136T | site-directed mutagenesis | Pseudoalteromonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudoalteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. | - |
isolated from antarctic ocean | - |
Pseudoalteromonas sp. AS-131 | - |
isolated from antarctic ocean | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21Star(DE3) to homogeneity by ATP affinity chromatography and dialysis | Pseudoalteromonas sp. |
Subunits | Comment | Organism |
---|---|---|
dimer | tertiary structure homology modelling of wild-type and mutant enzymes using the tetrameric structure of mutagenized HaNDK, PDB ID 3VGU | Pseudoalteromonas sp. |
Synonyms | Comment | Organism |
---|---|---|
ASNDK | - |
Pseudoalteromonas sp. |
nucleoside diphosphate kinase | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
recombinant enzyme | Pseudoalteromonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | decrease in Kcat/Km with increasing temperature from 20°C to 37°C, inactivation at 50°C | Pseudoalteromonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | degeneration during recombinant expression at 37°C, decrease in Kcat/Km with increasing temperature from 20°C to 37°C, Tm is 38°C, inactivation of purified enzyme at 50°C | Pseudoalteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudoalteromonas sp. |