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Literature summary for 2.7.4.8 extracted from

  • Agarwal, K.C.; Miech, R.P.; Parks, R.E.
    Guanylate kinases from human erythrocytes, hog brain, and rat liver (1978), Methods Enzymol., 51, 483-490.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Homo sapiens
5,5'-dithiobis(2-nitrobenzoate)
-
Rattus norvegicus
additional information no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate Homo sapiens
additional information no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate Rattus norvegicus
additional information no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate Sus scrofa
N-ethylmaleimide
-
Homo sapiens
N-ethylmaleimide
-
Rattus norvegicus
p-chloromercuribenzoic acid 1,4-dithiothreitol reverses Homo sapiens
p-chloromercuribenzoic acid 1,4-dithiothreitol reverses Rattus norvegicus
p-chloromercuribenzoic acid 1,4-dithiothreitol reverses Sus scrofa
p-hydroxymercuribenzoate
-
Homo sapiens
p-hydroxymercuribenzoate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
GMP pH 7.5, 30ºC Sus scrofa
0.01
-
GMP pH 7.5, 30ºC Rattus norvegicus
0.01
-
dGMP pH 7.5, 30ºC Rattus norvegicus
0.01
-
dGMP pH 7.5, 30ºC Sus scrofa
0.013
-
8-azaguanosine 5'-monophosphate pH 7.5, 30ºC Sus scrofa
0.018
-
GMP pH 7.5, 30ºC Homo sapiens
0.028
-
dGMP pH 7.5, 30ºC Rattus norvegicus
0.07
-
8-azaguanosine 5'-monophosphate pH 7.5, 30ºC Rattus norvegicus
0.074
-
dGMP pH 7.5, 30ºC Homo sapiens
0.091
-
8-azaguanosine 5'-monophosphate pH 7.5, 30ºC Homo sapiens
0.12
-
ATP pH 7.5, 30ºC Sus scrofa
0.18
-
ATP pH 7.5, 30ºC Rattus norvegicus
0.19
-
ATP pH 7.5, 30ºC Homo sapiens
2.1
-
6-thioguanosine 5'-monophosphate pH 7.5, 30ºC Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no activator Homo sapiens
Ca2+ no activator Rattus norvegicus
Ca2+ no activator Sus scrofa
K+ activation Homo sapiens
K+ activation Rattus norvegicus
K+ activation Sus scrofa
Li+ partial activity Sus scrofa
Mg2+
-
Homo sapiens
Mg2+
-
Rattus norvegicus
Mg2+
-
Sus scrofa
Mn2+ requirement Homo sapiens
Mn2+ requirement Rattus norvegicus
Mn2+ requirement Sus scrofa
additional information
-
Homo sapiens
additional information
-
Rattus norvegicus
additional information no activation by Sr2+ or Cs2+ Sus scrofa
Na+ activation Sus scrofa
NH4+ activation Homo sapiens
NH4+ activation Rattus norvegicus
NH4+ activation Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method that includes DEAE-cellulose and Sephadex-75 chromatography Rattus norvegicus
method that includes DEAE-cellulose and Sephadex-75 chromatography Sus scrofa
method that includes DEAE-cellulose, Sephadex-75 chromatography and isoelectric focusing Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain
-
Sus scrofa
-
erythrocyte
-
Homo sapiens
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
0°C, below, in 70% saturated ammonium sulfate, several years Homo sapiens
0°C, below, in 70% saturated ammonium sulfate, several years Rattus norvegicus
0°C, below, in 70% saturated ammonium sulfate, several years Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-thioguanosine 5'-monophosphate
-
Homo sapiens ADP + 8-thioguanosine 5'-diphosphate
-
?
ATP + 6-thioguanosine 5'-monophosphate
-
Rattus norvegicus ADP + 8-thioguanosine 5'-diphosphate
-
?
ATP + 6-thioguanosine 5'-monophosphate
-
Sus scrofa ADP + 8-thioguanosine 5'-diphosphate
-
?
ATP + 8-azaguanosine 5'-monophosphate
-
Homo sapiens ADP + 8-azaguanosine 5'-diphosphate
-
?
ATP + 8-azaguanosine 5'-monophosphate
-
Rattus norvegicus ADP + 8-azaguanosine 5'-diphosphate
-
?
ATP + 8-azaguanosine 5'-monophosphate
-
Sus scrofa ADP + 8-azaguanosine 5'-diphosphate
-
?
ATP + dGMP
-
Homo sapiens ADP + dGDP
-
r
ATP + dGMP
-
Rattus norvegicus ADP + dGDP
-
r
ATP + dGMP
-
Sus scrofa ADP + dGDP
-
r
ATP + GMP specificity Homo sapiens ADP + GDP
-
?
ATP + GMP specificity Rattus norvegicus ADP + GDP
-
?
ATP + GMP specificity Sus scrofa ADP + GDP
-
?
ATP + GMP nucleoside monophosphate binding site is highly specific for guanine moiety Homo sapiens ADP + GDP
-
?
ATP + GMP nucleoside monophosphate binding site is highly specific for guanine moiety Rattus norvegicus ADP + GDP
-
?
ATP + GMP nucleoside monophosphate binding site is highly specific for guanine moiety Sus scrofa ADP + GDP
-
?
ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Homo sapiens ADP + GDP
-
?
ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Rattus norvegicus ADP + GDP
-
?
ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Sus scrofa ADP + GDP
-
?
ATP + GMP 6-thio-IMP is no acceptor substrate Homo sapiens ADP + GDP
-
?
ATP + GMP 6-thio-IMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP 6-thio-IMP is no acceptor substrate Sus scrofa ADP + GDP
-
?
ATP + GMP XMP is no acceptor substrate Homo sapiens ADP + GDP
-
?
ATP + GMP XMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP XMP is no acceptor substrate Sus scrofa ADP + GDP
-
?
ATP + GMP AMP is no acceptor substrate Homo sapiens ADP + GDP
-
?
ATP + GMP AMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP AMP is no acceptor substrate Sus scrofa ADP + GDP
-
?
ATP + GMP CMP, UMP are no acceptor substrates Homo sapiens ADP + GDP
-
?
ATP + GMP CMP, UMP are no acceptor substrates Rattus norvegicus ADP + GDP
-
?
ATP + GMP CMP, UMP are no acceptor substrates Sus scrofa ADP + GDP
-
?
ATP + IMP very poor substrate Homo sapiens ADP + IDP
-
?
dATP + dGMP
-
Homo sapiens dADP + dGDP
-
?
dATP + dGMP
-
Rattus norvegicus dADP + dGDP
-
?
dATP + dGMP
-
Sus scrofa dADP + dGDP
-
?
dATP + GMP
-
Homo sapiens dADP + GDP
-
?
dATP + GMP
-
Rattus norvegicus dADP + GDP
-
?
dATP + GMP
-
Sus scrofa dADP + GDP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens
30
-
assay at Rattus norvegicus
30
-
assay at Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
at least 15 min stable from pH 5.5-8.5 Sus scrofa
50
-
10 min, inactivation Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 at least 15 min stable at 30°C Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus 3 isoelectric variants, predominant form: pI 4.9, agarose gel electrophoresis and isoelectric focusing 4.8 4.7
Homo sapiens 4 isoelectric variants, agarose gel electrophoresis and isoelectric focusing
-
4.9