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Literature summary for 2.7.4.8 extracted from
- Purification and properties of guanylate kinase from bakers yeast (1981), Biochim. Biophys. Acta, 662, 165-167.
General Stability
| General Stability | Organism |
|---|---|
| 20% glycerol stabilizes: 90% original activity retained | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
GMP | - |
Saccharomyces cerevisiae |  |
| 0.5 | - |
ATP | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ | Saccharomyces cerevisiae | |
| Mg2+ | - |
Saccharomyces cerevisiae | |
| Mn2+ | requirement | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method that includes DEAE-cellulose, hydroxyapatite and Sephadex-75 chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.6 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, at pH-values below 4 or above 9, inactivation within 48 h | Saccharomyces cerevisiae |
| 4°C, in 20% glycerol, at least 1 month | Saccharomyces cerevisiae |
| glycerol, 20%, stabilizes labile enzyme during storage | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GMP | best substrates | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | IMP is no acceptor substrate | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | XMP is no acceptor substrate | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | AMP is no acceptor substrate | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | CMP, UMP are no acceptor substrates | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
t1/2: 10 min | Saccharomyces cerevisiae |
| 60 | - |
10 min, 90% loss of activity | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 7.5 | about half-maximal activity at pH 5.2 and 7.5 | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | 4°C, inactivation within 48 h outside this range | Saccharomyces cerevisiae |
| 7.5 | - |
most stable at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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