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Literature summary for 2.7.4.8 extracted from
- Kinetic and thermodynamic characterizations of yeast guanylate kinase (1996), J. Biol. Chem., 271, 28038-28044.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S80A | sluggish enzyme | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | - |
Saccharomyces cerevisiae |  |
| GMP | non competitive with respect to MgATP2- because of the formation of an abortive complex guanylate kinase-MgATP2-GMP | Saccharomyces cerevisiae | |
| MgADP- | - |
Saccharomyces cerevisiae | |
| MgATP2- | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
MgADP- | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.091 | - |
GMP | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.097 | - |
GDP | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.2 | - |
MgATP2- | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.23 | - |
MgATP2- | pH 7.5, 25ºC, S80A mutant | Saccharomyces cerevisiae | |
| 0.38 | - |
dGMP | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.5 | - |
GMP | pH 7.5, 25ºC, S80A mutant | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GMP | - |
Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | - |
Saccharomyces cerevisiae | ADP + GDP | - |
r | |
| GMP + MgATP2- | - |
Saccharomyces cerevisiae | MgADP- + GDP | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51 | - |
dGMP | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 90 | - |
ATP | pH 7.7, 25ºC, reverse reaction | Saccharomyces cerevisiae | |
| 394 | - |
GMP | pH 7.7, 25ºC, forward reaction | Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
GMP | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.037 | - |
MgADP- | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.08 | - |
MgATP2- | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
| 0.215 | - |
GDP | pH 7.5, 25ºC | Saccharomyces cerevisiae | |
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