Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S35N/V168F | the mutations significantly suppress enzyme catalytic activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
GMP | wild type enzyme, in 100 mM TrisHCl buffer pH 7.5, 4 mM ATP, 10 mM MgCl2, 1 mM dithiothreitol, 100 mM KCl, at 30°C | Homo sapiens | |
0.27 | - |
GMP | mutant S35N/V168F, in 100 mM TrisHCl buffer pH 7.5, 4 mM ATP, 10 mM MgCl2, 1 mM dithiothreitol, 100 mM KCl, at 30°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 22000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CCRF-CEM cell | - |
Homo sapiens | - |
T-lymphoblast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GMP | - |
Homo sapiens | ADP + GDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GUK | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | there is an approximately 40% drop in guanylate kinase mRNA expression in cell lines resistant to 9-[2-(phosphonomethoxyethyl)guanine] and 9-[2-(phosphonomethoxyethyl)diaminopurine] | down |
General Information | Comment | Organism |
---|---|---|
physiological function | guanylate kinase is the sole molecular target for the development of acquired resistance to the cytotoxic nucleotide 9-[2-(phosphonomethoxyethyl)]-guanine | Homo sapiens |