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Literature summary for 2.7.4.8 extracted from

  • Mertlikova-Kaiserova, H.; Rumlova, M.; Tloustova, E.; Prochazkova, E.; Holy, A.; Votruba, I.
    Point mutations in human guanylate kinase account for acquired resistance to anticancer nucleotide analogue PMEG (2011), Biochem. Pharmacol., 82, 131-138.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
S35N/V168F the mutations significantly suppress enzyme catalytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
GMP wild type enzyme, in 100 mM Tris–HCl buffer pH 7.5, 4 mM ATP, 10 mM MgCl2, 1 mM dithiothreitol, 100 mM KCl, at 30°C Homo sapiens
0.27
-
GMP mutant S35N/V168F, in 100 mM Tris–HCl buffer pH 7.5, 4 mM ATP, 10 mM MgCl2, 1 mM dithiothreitol, 100 mM KCl, at 30°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
x * 22000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CCRF-CEM cell
-
Homo sapiens
-
T-lymphoblast
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GMP
-
Homo sapiens ADP + GDP
-
?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GUK
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens there is an approximately 40% drop in guanylate kinase mRNA expression in cell lines resistant to 9-[2-(phosphonomethoxyethyl)guanine] and 9-[2-(phosphonomethoxyethyl)diaminopurine] down

General Information

General Information Comment Organism
physiological function guanylate kinase is the sole molecular target for the development of acquired resistance to the cytotoxic nucleotide 9-[2-(phosphonomethoxyethyl)]-guanine Homo sapiens