General Stability | Organism |
---|---|
dTDP and dTTP are effective as enzyme stabilizer | Mus musculus |
the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.4 mM | Mus musculus | |
ADP | 100 nM, 64% inhibition | Mus musculus | |
Ca2+ | - |
Mus musculus | |
CH2ATP | 100 nM, 23% inhibition | Mus musculus | |
dADP | 100 nM, 57% inhibition | Mus musculus | |
dATP | 100 nM, 15% inhibition | Mus musculus | |
dGTP | 100 nM, 12% inhibition | Mus musculus | |
dTDP | 100 nM, 39% inhibition | Mus musculus | |
GDP | 100 nM, 21% inhibition | Mus musculus | |
Mn2+ | slight inhibition | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
dTMP | pH 7.4, 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, concentration range for maximal activity is From Mg:ATP ratios of 1 to 3 with an optimum around 1.3 to 1.5 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma ascites cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.76 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTMP | - |
Mus musculus | ADP + dTDP | - |
r | |
ATP + dUMP | 23% of the activity with dTMP | Mus musculus | ADP + dUDP | - |
r | |
dATP + dTMP | 70.1% of the activity with ATP | Mus musculus | dADP + dTDP | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
ADP | pH 7.4, 37°C | Mus musculus |