Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Yersinia pestis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
TMP | pH 7.4, 30°C | Escherichia coli | |
0.045 | - |
TMP | pH 7.4, 30°C | Yersinia pestis | |
0.08 | - |
5-bromo-2'-deoxyuridine 5'-monophosphate | pH 7.4, 30°C | Escherichia coli | |
0.09 | - |
3'-azido-3'-deoxythymidine 5'-monophosphate | pH 7.4, 30°C | Yersinia pestis | |
0.1 | - |
5-bromo-2'-deoxyuridine 5'-monophosphate | pH 7.4, 30°C | Yersinia pestis | |
0.17 | - |
3'-azido-3'-deoxythymidine 5'-monophosphate | pH 7.4, 30°C | Escherichia coli | |
0.17 | - |
3'-azido-3'-deoxythymidine 5'-monophosphate | pH 7.4, 30°C | Yersinia pestis | |
0.22 | - |
2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate | pH 7.4, 30°C | Escherichia coli | |
0.27 | - |
2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate | pH 7.4, 30°C | Yersinia pestis | |
2.5 | - |
dUMP | pH 7.4, 30°C | Escherichia coli | |
3.8 | - |
dUMP | pH 7.4, 30°C | Yersinia pestis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | key enzyme in nucleotide synthesis | ? | - |
? | |
additional information | Yersinia pestis | key enzyme in nucleotide synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Yersinia pestis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Yersinia pestis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate | - |
Escherichia coli | ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate | - |
? | |
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate | - |
Yersinia pestis | ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate | - |
? | |
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate | good substrate | Escherichia coli | ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate | - |
? | |
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate | Vmax is 100 times lower than with dTMP | Yersinia pestis | ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate | - |
? | |
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate | - |
Escherichia coli | ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate | - |
? | |
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate | - |
Yersinia pestis | ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate | - |
? | |
ATP + dTMP | - |
Escherichia coli | ADP + dTDP | - |
? | |
ATP + dTMP | - |
Yersinia pestis | ADP + dTDP | - |
? | |
ATP + dUMP | - |
Escherichia coli | ADP + dUDP | - |
? | |
ATP + dUMP | - |
Yersinia pestis | ADP + dUDP | - |
? | |
CTP + dTMP | - |
Escherichia coli | CDP + dTDP | - |
? | |
CTP + dTMP | - |
Yersinia pestis | CDP + dTDP | - |
? | |
dATP + dTMP | - |
Escherichia coli | dADP + dTDP | - |
? | |
dATP + dTMP | - |
Yersinia pestis | dADP + dTDP | - |
? | |
GTP + dTMP | - |
Escherichia coli | GDP + dTDP | - |
? | |
GTP + dTMP | - |
Yersinia pestis | GDP + dTDP | - |
? | |
ITP + dTMP | 1% of the activity with ATP and TMP | Yersinia pestis | IDP + dTDP | - |
? | |
ITP + dTMP | 2% of the activity with ATP and TMP | Escherichia coli | IDP + dTDP | - |
? | |
additional information | key enzyme in nucleotide synthesis | Escherichia coli | ? | - |
? | |
additional information | key enzyme in nucleotide synthesis | Yersinia pestis | ? | - |
? | |
UTP + dTMP | 2.5% of the activity with ATP and TMP | Yersinia pestis | UDP + dTDP | - |
? | |
UTP + dTMP | 13% of the activity with ATP and TMP | Escherichia coli | UDP + dTDP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the denaturation temperature of the enzyme increase by 2.9°C in the presence of 5 mM ATP and by 8°C in the presence of 5 mM dTMP | Yersinia pestis |
49 | - |
Tm-value | Yersinia pestis |
57 | - |
Tm-value | Escherichia coli |