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Literature summary for 2.7.4.9 extracted from

  • Chenal-FRancisque, V.; Tourneux, L.; carniel, E.; Christova, P.; de la Sierra, I.; Barzu, O.; Gilles, A.M.
    The highly similar TMP kinase of Yersinia pestis and Escherichia coli differ markedly in their ATTMP phosphorylating activity (1999), Eur. J. Biochem., 265, 112-119.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Yersinia pestis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
TMP pH 7.4, 30°C Escherichia coli
0.045
-
TMP pH 7.4, 30°C Yersinia pestis
0.08
-
5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C Escherichia coli
0.09
-
3'-azido-3'-deoxythymidine 5'-monophosphate pH 7.4, 30°C Yersinia pestis
0.1
-
5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C Yersinia pestis
0.17
-
3'-azido-3'-deoxythymidine 5'-monophosphate pH 7.4, 30°C Escherichia coli
0.17
-
3'-azido-3'-deoxythymidine 5'-monophosphate pH 7.4, 30°C Yersinia pestis
0.22
-
2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate pH 7.4, 30°C Escherichia coli
0.27
-
2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate pH 7.4, 30°C Yersinia pestis
2.5
-
dUMP pH 7.4, 30°C Escherichia coli
3.8
-
dUMP pH 7.4, 30°C Yersinia pestis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli key enzyme in nucleotide synthesis ?
-
?
additional information Yersinia pestis key enzyme in nucleotide synthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Yersinia pestis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Yersinia pestis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate
-
Escherichia coli ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate
-
Yersinia pestis ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate good substrate Escherichia coli ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate Vmax is 100 times lower than with dTMP Yersinia pestis ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
?
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate
-
Escherichia coli ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate
-
?
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate
-
Yersinia pestis ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate
-
?
ATP + dTMP
-
Escherichia coli ADP + dTDP
-
?
ATP + dTMP
-
Yersinia pestis ADP + dTDP
-
?
ATP + dUMP
-
Escherichia coli ADP + dUDP
-
?
ATP + dUMP
-
Yersinia pestis ADP + dUDP
-
?
CTP + dTMP
-
Escherichia coli CDP + dTDP
-
?
CTP + dTMP
-
Yersinia pestis CDP + dTDP
-
?
dATP + dTMP
-
Escherichia coli dADP + dTDP
-
?
dATP + dTMP
-
Yersinia pestis dADP + dTDP
-
?
GTP + dTMP
-
Escherichia coli GDP + dTDP
-
?
GTP + dTMP
-
Yersinia pestis GDP + dTDP
-
?
ITP + dTMP 1% of the activity with ATP and TMP Yersinia pestis IDP + dTDP
-
?
ITP + dTMP 2% of the activity with ATP and TMP Escherichia coli IDP + dTDP
-
?
additional information key enzyme in nucleotide synthesis Escherichia coli ?
-
?
additional information key enzyme in nucleotide synthesis Yersinia pestis ?
-
?
UTP + dTMP 2.5% of the activity with ATP and TMP Yersinia pestis UDP + dTDP
-
?
UTP + dTMP 13% of the activity with ATP and TMP Escherichia coli UDP + dTDP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the denaturation temperature of the enzyme increase by 2.9°C in the presence of 5 mM ATP and by 8°C in the presence of 5 mM dTMP Yersinia pestis
49
-
Tm-value Yersinia pestis
57
-
Tm-value Escherichia coli