Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
For the His-tagged SaTMK crystals, data is collected on beamline ID 14.1 at the ESRF (Grenoble, France). | Staphylococcus aureus |
The data for the untagged SaTMK crystals is collected using Cu Ka radiation from a Rigaku MicroMax 007 generator equipped with aMAR345 image plate. | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymidine monophosphate + ATP | Staphylococcus aureus | Thymidylate kinase (TMK) (E.C. 2.7.4.9) is a member of the NMP kinase family and catalyzes the phosphoryl transfer from the preferred phosphoryl donor, ATP, to thymidine monophosphate (TMP), yielding thymidine diphosphate (TDP). The TMK reaction is positioned at the junction of the de novo and salvage pathway of thymidine triphosphate (dTTP) synthesis, with TMK being the last specific enzyme for dTTP synthesis. | thymidine diphosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Zn2+ charched sepharose column, hydroxyapalite column | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymidine monophosphate + ATP | Thymidylate kinase (TMK) (E.C. 2.7.4.9) is a member of the NMP kinase family and catalyzes the phosphoryl transfer from the preferred phosphoryl donor, ATP, to thymidine monophosphate (TMP), yielding thymidine diphosphate (TDP). The TMK reaction is positioned at the junction of the de novo and salvage pathway of thymidine triphosphate (dTTP) synthesis, with TMK being the last specific enzyme for dTTP synthesis. | Staphylococcus aureus | thymidine diphosphate + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S. aureus thymidylate kinase | - |
Staphylococcus aureus |