Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | activity of the enzyme is independent of phosphate. Without effect up to 80 mM | Spinacia oleracea |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate | competitive with respect to ATP and noncompetitive with respect to D-ribose 5-phosphate | Spinacia oleracea | |
ADP | competitive inhibition to ATP | Spinacia oleracea | |
AMP | non competitive with respect to D-ribose 5-phosphate and ATP | Spinacia oleracea | |
UTP | substrate inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
D-ribose 5-phosphate | pH conditions not mentioned, 37ºC | Spinacia oleracea | |
0.077 | - |
ATP | pH conditions not mentioned, 37ºC | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activity observed | Spinacia oleracea | |
Mg2+ | requirement | Spinacia oleracea | |
Mg2+ | required to form a complex with ATP and as a free cation | Spinacia oleracea | |
Mn2+ | 25-30% of the activity with Mg2+ | Spinacia oleracea | |
additional information | no activity observed with Ca2+ | Spinacia oleracea | |
additional information | no activity observed with Cd2+, Cu2+, Fe2+ and Ni2+ | Spinacia oleracea | |
Zn2+ | no activity observed | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Spinacia oleracea | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
streptomycin sulfate and ammonium sulfate precipitation, chromatography on Dyematrex Gel Blue B and DE52 | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction | Spinacia oleracea | |
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | sequential kinetic mechanism | Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20ºC, Tris/HCl buffer 50 mM, pH 7.6, 50% glycerol, stable | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Spinacia oleracea | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
CTP + D-ribose 5-phosphate | - |
Spinacia oleracea | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
dATP + D-ribose 5-phosphate | - |
Spinacia oleracea | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
GTP + D-ribose 5-phosphate | - |
Spinacia oleracea | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
UTP + D-ribose 5-phosphate | - |
Spinacia oleracea | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
37ºC, in Tris/ClH buffer | Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
ADP | pH conditions not mentioned, 37ºC, ATP as substrate, Kis value | Spinacia oleracea | |
0.16 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH conditions not mentioned, 37ºC, ATP as substrate, Kis value | Spinacia oleracea | |
0.33 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kis value | Spinacia oleracea | |
0.38 | - |
AMP | pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kis value | Spinacia oleracea | |
0.43 | - |
AMP | pH conditions not mentioned, 37ºC, ATP as substrate, Kis value | Spinacia oleracea | |
2.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kii value | Spinacia oleracea | |
3.9 | - |
AMP | pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kii value | Spinacia oleracea | |
5.6 | - |
AMP | pH conditions not mentioned, 37ºC, ATP as substrate, Kii value | Spinacia oleracea |