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Literature summary for 2.7.6.1 extracted from

  • Krath, B.N.; Hove-Jensen, B.
    Implications of secondary structure prediction and amino acid sequence comparison of class I and class II phosphoribosyl diphosphate synthases on catalysis, regulation, and quaternary structure (2001), Protein Sci., 10, 2317-2324.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphate activity of the enzyme is independent of phosphate. Without effect up to 80 mM Spinacia oleracea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
5-phospho-alpha-D-ribose 1-diphosphate competitive with respect to ATP and noncompetitive with respect to D-ribose 5-phosphate Spinacia oleracea
ADP competitive inhibition to ATP Spinacia oleracea
AMP non competitive with respect to D-ribose 5-phosphate and ATP Spinacia oleracea
UTP substrate inhibition Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
D-ribose 5-phosphate pH conditions not mentioned, 37ºC Spinacia oleracea
0.077
-
ATP pH conditions not mentioned, 37ºC Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activity observed Spinacia oleracea
Mg2+ requirement Spinacia oleracea
Mg2+ required to form a complex with ATP and as a free cation Spinacia oleracea
Mn2+ 25-30% of the activity with Mg2+ Spinacia oleracea
additional information no activity observed with Ca2+ Spinacia oleracea
additional information no activity observed with Cd2+, Cu2+, Fe2+ and Ni2+ Spinacia oleracea
Zn2+ no activity observed Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribose 5-phosphate Spinacia oleracea
-
AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
spinach
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin sulfate and ammonium sulfate precipitation, chromatography on Dyematrex Gel Blue B and DE52 Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Spinacia oleracea
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate sequential kinetic mechanism Spinacia oleracea

Storage Stability

Storage Stability Organism
-20ºC, Tris/HCl buffer 50 mM, pH 7.6, 50% glycerol, stable Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Spinacia oleracea AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
CTP + D-ribose 5-phosphate
-
Spinacia oleracea CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
dATP + D-ribose 5-phosphate
-
Spinacia oleracea dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
GTP + D-ribose 5-phosphate
-
Spinacia oleracea GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
UTP + D-ribose 5-phosphate
-
Spinacia oleracea UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
37ºC, in Tris/ClH buffer Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
ADP pH conditions not mentioned, 37ºC, ATP as substrate, Kis value Spinacia oleracea
0.16
-
5-phospho-alpha-D-ribose 1-diphosphate pH conditions not mentioned, 37ºC, ATP as substrate, Kis value Spinacia oleracea
0.33
-
5-phospho-alpha-D-ribose 1-diphosphate pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kis value Spinacia oleracea
0.38
-
AMP pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kis value Spinacia oleracea
0.43
-
AMP pH conditions not mentioned, 37ºC, ATP as substrate, Kis value Spinacia oleracea
2.2
-
5-phospho-alpha-D-ribose 1-diphosphate pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kii value Spinacia oleracea
3.9
-
AMP pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kii value Spinacia oleracea
5.6
-
AMP pH conditions not mentioned, 37ºC, ATP as substrate, Kii value Spinacia oleracea