Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | requirement | Hevea brasiliensis | |
additional information | no other sulfhydryl group protector or antioxidant can effectively replace DTT | Hevea brasiliensis | |
phosphate | activation at 1 mM of D-ribose 5-phosphate, maximal activity from 10 mM of D-ribose 5-phosphate | Hevea brasiliensis | |
sulfate | no activity observed | Hevea brasiliensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate | - |
Hevea brasiliensis | |
adenosine | no inhibition | Hevea brasiliensis | |
ADP | no inhibition | Hevea brasiliensis | |
AMP | no inhibition | Hevea brasiliensis | |
ATP | no inhibition | Hevea brasiliensis | |
D-fructose 1,6-diphosphate | no inhibition | Hevea brasiliensis | |
GDP | no inhibition | Hevea brasiliensis | |
glyceraldehyde 3-phosphate | no inhibition | Hevea brasiliensis | |
GMP | no inhibition | Hevea brasiliensis | |
GTP | no inhibition | Hevea brasiliensis | |
guanosine | no inhibition | Hevea brasiliensis | |
L-histidine | no inhibition | Hevea brasiliensis | |
L-tryptophan | no inhibition | Hevea brasiliensis | |
Mn2+ | complete inhibition above 0.4 mM | Hevea brasiliensis | |
additional information | D-glucose-6-phosphate have no effects on the enzyme activity; dihydroxyacetone phosphate, D-glucose-1-phosphate, fructose-6-phosphate, ribose, sucrose, glutamine, glutamic acid, aspartic acid, alanine, alpha-ketoglutarate, pyruvate, citrate, malate, fumarate, succinate, cytidine, uridine, adenine, orotate, guanine, cytosine and xanthine have no effects on the enzyme activity | Hevea brasiliensis | |
phosphate | inhibition at low concentrations of D-ribose 5-phosphate | Hevea brasiliensis | |
UDP | no inhibition | Hevea brasiliensis | |
UMP | no inhibition | Hevea brasiliensis | |
UTP | no inhibition | Hevea brasiliensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-ribose 5-phosphate | pH 7.5, 37ºC | Hevea brasiliensis | |
0.2 | - |
ATP | pH 7.5, 37ºC | Hevea brasiliensis | |
1.5 | - |
Mg2+ | pH 7.5, 37ºC | Hevea brasiliensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | also known as latex in specialized cells | Hevea brasiliensis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activity observed | Hevea brasiliensis | |
Mg2+ | requirement | Hevea brasiliensis | |
Mn2+ | no activity observed | Hevea brasiliensis | |
additional information | K+, Na+, Fe2+ Cu2+ and Ca2+ have no notable effects on the enzyme activity | Hevea brasiliensis | |
Zn2+ | no activity observed | Hevea brasiliensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Hevea brasiliensis |
200000 | - |
gel filtration | Hevea brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | - |
rubber tree | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, chromatography on Sephacryl S300 HR and on Protein-Pak DEAE 40 HR and affinity chromatography | Hevea brasiliensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
laticifer | - |
Hevea brasiliensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Hevea brasiliensis | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
CTP + D-ribose 5-phosphate | does not serve as substrate | Hevea brasiliensis | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
GTP + D-ribose 5-phosphate | does not serve as substrate | Hevea brasiliensis | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
ITP + D-ribose 5-phosphate | does not serve as substrate | Hevea brasiliensis | IMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UTP + D-ribose 5-phosphate | does not serve as substrate | Hevea brasiliensis | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Hevea brasiliensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hevea brasiliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Hevea brasiliensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Mn2+ | pH 7.5, 37ºC | Hevea brasiliensis | |
0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37ºC | Hevea brasiliensis |