Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Bacillus amyloliquefaciens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus amyloliquefaciens |
Protein Variants | Comment | Organism |
---|---|---|
D58H | the mutant exhibits nearly identical sensitivity to ADP and GDP as the wild-type protein and has a notably greater phosphate requirement for activation | Bacillus amyloliquefaciens |
L135I | the mutation releases the enzyme from ADP and GDP inhibition and significantly increases its sensitivity to inorganic phosphate activation | Bacillus amyloliquefaciens |
N120S | the mutation releases the enzyme from ADP and GDP inhibition and significantly increases its sensitivity to inorganic phosphate activation | Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Bacillus amyloliquefaciens | |
GDP | - |
Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.06 | - |
ATP | mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.083 | - |
ATP | mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.094 | - |
ATP | mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.105 | - |
D-ribose 5-phosphate | wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.157 | - |
D-ribose 5-phosphate | mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.18 | - |
D-ribose 5-phosphate | mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | |
0.189 | - |
D-ribose 5-phosphate | mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
186000 | - |
gel filtration | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | E7EAU9 | - |
- |
Bacillus amyloliquefaciens IAM 1523 | E7EAU9 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Sephadex G-25 gel filtration | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Bacillus amyloliquefaciens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
ATP + D-ribose 5-phosphate | - |
Bacillus amyloliquefaciens IAM 1523 | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosyl pyrophosphate synthetase | - |
Bacillus amyloliquefaciens |
PRS | - |
Bacillus amyloliquefaciens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.07 | - |
mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | ADP | |
0.08 | - |
wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | ADP | |
0.44 | - |
mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | ADP | |
0.45 | - |
wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | GDP | |
0.51 | - |
mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | GDP | |
0.84 | - |
mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | ADP | |
1 | - |
IC50 above 1 mM, mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | GDP | |
1 | - |
IC50 above 1 mM, mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C | Bacillus amyloliquefaciens | GDP |