Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | the enzyme requires the presence of phosphate at a concentration of 50 mM for full activation | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41 cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | strong inhibitor | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
D-ribose 5-phosphate | at pH 7.5 and 37°C | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mycobacterium tuberculosis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis | |
Mn2+ | required | Mycobacterium tuberculosis | |
additional information | Mn2+ and Mg2+ cannot be replaced by Ca2+ | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl-beta,gamma-imidodiphosphate + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | ? | - |
? | |
5'-adenylyl-beta,gamma-imidodiphosphate + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
ATP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
ir | |
ATP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis H37Rv | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosyl-1-pyrophosphate synthetase | - |
Mycobacterium tuberculosis |
PRPP synthetase | - |
Mycobacterium tuberculosis |
PrsA | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | the enzyme shows activity across a broad range of pH levels (4.5-9.5) and is only found to be inactive at high pH 10.5 | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.522 | - |
ADP | at pH 7.5 and 37°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7430 | - |
D-ribose 5-phosphate | at pH 7.5 and 37°C | Mycobacterium tuberculosis |