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Literature summary for 2.7.6.2 extracted from

  • Sanemori, H.; Kawasaki, T.
    Purification and properties of thiamine pyrophosphokinase in Paracoccus denitrificans (1980), J. Biochem., 88, 223-230.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Chloroethylthiamine weak Paracoccus denitrificans
iodoacetamide 3.3 mM, 23.3% inhibition, activity is recovered to approximately 90% on incubation with a 10fold excess of dithiothreitol Paracoccus denitrificans
oxythiamine weak Paracoccus denitrificans
PCMB 1 mM, 55.1% inhibition, activity is recovered to approximately 90% on incubation with a 10fold excess of dithiothreitol Paracoccus denitrificans
pyrithiamine
-
Paracoccus denitrificans
thiamine diphosphate
-
Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
thiamine pH 9.0, 37°C Paracoccus denitrificans
0.38
-
ATP pH 9.0, 37°C Paracoccus denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Paracoccus denitrificans
96000
-
aggregate of the functional dimer, equilibrium sedimentation Paracoccus denitrificans
230000
-
2 * 230000, SDS-PAGE Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-
Paracoccus denitrificans 12442
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Paracoccus denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0235
-
-
Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thiamine
-
Paracoccus denitrificans AMP + thiamine diphosphate
-
?
ATP + thiamine
-
Paracoccus denitrificans 12442 AMP + thiamine diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 230000, SDS-PAGE Paracoccus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Paracoccus denitrificans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
pyrithiamine
-
Paracoccus denitrificans