Cloned (Comment) | Organism |
---|---|
isozymes TPK1 and TPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21, expression of fluorescent tagged isozymes in cytosol of mesophyll protoplasts | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a double knockout mutant lacking isozymes TPK1 and TPK2, the mutant shows severely depressed levels of TPP and elevated levels of free thiamine compared to the wild-type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00096 | - |
thiamine | AtTPK1 | Arabidopsis thaliana | |
0.00096 | - |
thiamine | pH 8.0, 37°C, isozyme TPK1 | Arabidopsis thaliana | |
0.00128 | - |
thiamine | AtTPK2 | Arabidopsis thaliana | |
0.00128 | - |
thiamine | pH 8.0, 37°C, isozyme TPK2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
cytosol | isozymes TPK1 and TPK2 | Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thiamine | Arabidopsis thaliana | thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis | AMP + thiamine diphosphate | - |
? | |
thiamin + ATP | Arabidopsis thaliana | - |
thiamindiphosphate + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | - |
isozymes TPK1 and TPK2 | - |
Arabidopsis thaliana | F4IV16 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | isozymes TPK1 and TPK2 are biochemically redundant cytosolic proteins that are similarly expressed throughout different plant tissues | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thiamine | thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis | Arabidopsis thaliana | AMP + thiamine diphosphate | - |
? | |
ATP + thiamine | no TMP kinase activity is shown by either isozymes TPK1 or TPK2 | Arabidopsis thaliana | AMP + thiamine diphosphate | - |
? | |
thiamin + ATP | - |
Arabidopsis thaliana | thiamindiphosphate + AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At1g02880 (AtTPK1) | - |
Arabidopsis thaliana |
At2g44750 (AtTPK2) | - |
Arabidopsis thaliana |
thiamin pyrophosphokinase | - |
Arabidopsis thaliana |
TPK | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
AtTPK2 | Arabidopsis thaliana |
37 | - |
isozyme TPK2 | Arabidopsis thaliana |
45 | - |
AtTPK1 | Arabidopsis thaliana |
45 | - |
isozyme TPK1 | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | 37°C: optimal activity of TPK2, 50°C: TPK2 is completely inactive | Arabidopsis thaliana |
45 | 55 | TPK1 | Arabidopsis thaliana |
50 | - |
AtTPK2 is completely inactive | Arabidopsis thaliana |
55 | - |
AtTPK1 is still active | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
inactivation of isozyme TPK2 | Arabidopsis thaliana |
55 | - |
isozyme TPK1 shows remaining activity | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arabidopsis thaliana |
7 | - |
isozymes TPK1 or TPK2 | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |