Literature summary for 2.7.6.3 extracted from

Storozhenko, S.; Navarrete, O.; Ravanel, S.; De Brouwer, V.; Chaerle, P.; Zhang, G.F.; Bastien, O.; Lambert, W.; Rebeille, F.; Van Der Straeten, D.
Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response (2007), J. Biol. Chem., 282, 10749-10761.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	expression of cytHPPK/DHPS mRNA is induced by salt stress	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Saccharomyces cerevisiae	Arabidopsis thaliana
isozyme cytHPPK/DHPS DNA and amino acid sequence determination and analysis, and phylogenetic analysis, transient expression of GFP-tagged isozyme cytHPPK/DHPS in the cytosol of Arabidopsis thaliana protoplasts using transfection via Agrobacterium tumefaciens strain GV3101/pMP90, functional complementation of a Saccharomyces cerevisiae mutant, that lacks the enzyme activity, by expression of isozyme cytHPPK/DHPS, expression and subcloning of N-terminally His6-tagged isozyme cytHPPK/DHPS in Escherichia coli strains DH-5alpha and BL-21AI, co-expression with GroESL chaperones	Arabidopsis thaliana

Cloned (Comment)

Organism

expression in Escherichia coli and Saccharomyces cerevisiae

Arabidopsis thaliana

isozyme cytHPPK/DHPS DNA and amino acid sequence determination and analysis, and phylogenetic analysis, transient expression of GFP-tagged isozyme cytHPPK/DHPS in the cytosol of Arabidopsis thaliana protoplasts using transfection via Agrobacterium tumefaciens strain GV3101/pMP90, functional complementation of a Saccharomyces cerevisiae mutant, that lacks the enzyme activity, by expression of isozyme cytHPPK/DHPS, expression and subcloning of N-terminally His6-tagged isozyme cytHPPK/DHPS in Escherichia coli strains DH-5alpha and BL-21AI, co-expression with GroESL chaperones

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a T-DNA insertion mutant in the cytHPPK/DHPS gene, resulting in lower germination rates as compared with the wild-type upon application of oxidative and osmotic stress, phenotype, overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
cytosol	isozyme cytHPPK/DHPS lacking a potential transit peptide	Arabidopsis thaliana	5829	-
mitochondrion	-	Arabidopsis thaliana	5739	-
mitochondrion	mitHPPK/DHPS	Arabidopsis thaliana	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54053	-	x * 54053, sequence calculation	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-hydroxymethyl-7,8-dihydropterin + ATP	Arabidopsis thaliana	tetrahydrofolate biosynthesis	6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP	-	?
ATP + 6-hydroxymethyl-7,8-dihydropterin	Arabidopsis thaliana	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview	AMP + (7,8-dihydropterin-6-yl)methyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Arabidopsis thaliana	Q1ENB6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged isozyme cytHPPK/DHPS from Escherichia coli strains DH-5alpha and BL-21AI	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	ubiquitous expression of isozyme mitHPPK/DHPS	Arabidopsis thaliana	-
seed	developing, isozyme cytHPPK/DHPS gene is exclusively expressed in developing seeds, histochemical analysis of a transgenic cytHPPK/DHPS promoter-GUS line	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
6-hydroxymethyl-7,8-dihydropterin + ATP	tetrahydrofolate biosynthesis	Arabidopsis thaliana	6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP	-	?
ATP + 6-hydroxymethyl-7,8-dihydropterin	-	Arabidopsis thaliana	AMP + (7,8-dihydropterin-6-yl)methyl diphosphate	-	?
ATP + 6-hydroxymethyl-7,8-dihydropterin	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview	Arabidopsis thaliana	AMP + (7,8-dihydropterin-6-yl)methyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 54053, sequence calculation	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK)	-	Arabidopsis thaliana
cytHPPK/DHPS	-	Arabidopsis thaliana
hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase	-	Arabidopsis thaliana
hydroxymethyldihydropterin pyrophosphokinase (HPPK)	-	Arabidopsis thaliana
mitHPPK/DHPS	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Arabidopsis thaliana