Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Mycobacterium smegmatis | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C633A | 20fold decrease in activity | Mycobacterium tuberculosis |
D632A | 3.5fold decrease in activity | Mycobacterium tuberculosis |
D81A | loss of hydrolytic activity with retention of synthesis | Mycobacterium tuberculosis |
G241E | loss of synthetic activity and retention of hydrolysis | Mycobacterium tuberculosis |
H344Y | loss of synthetic activity and retention of hydrolysis | Mycobacterium tuberculosis |
H80A | loss of hydrolytic activity with retention of synthesis | Mycobacterium tuberculosis |
additional information | fragments 1-203 (22.7 kDa) and 1-181 (20.1 kDa) possess hydrolytic activity but are incapable of synthesis activity, fragment 1-156 (17.3 kDa) is not capable of either synthesis or hydolytic activity, relative acticity of fragment 1-181 decreases approximately 130fold compared to that of the wild type, fragment 87-394 (35.1 kDa) has only synthesis activity, fragment 1-394 (44.6 kDa) and 1-450 are capable of synthesis and hydrolysis, the trimer state of fragment 1-394 appears to be a catalytically less efficient state than the monomer state, fragment 395-738 is devoid of any activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
GTP | full length enzyme + tRNA + ribosomes + poly(U) | Mycobacterium tuberculosis | |
0.67 | - |
GTP | fragment 1-394 + tRNA | Mycobacterium tuberculosis | |
0.67 | - |
GTP | fragment 1-394 + tRNA + ribosomes + poly(U) | Mycobacterium tuberculosis | |
0.67 | - |
GTP | fragment 1-394 monomer | Mycobacterium tuberculosis | |
0.7 | - |
GTP | full length enzyme + tRNA | Mycobacterium tuberculosis | |
0.78 | - |
GTP | fragment 87-394 | Mycobacterium tuberculosis | |
0.78 | - |
GTP | fragment 87-394 + tRNA | Mycobacterium tuberculosis | |
0.78 | - |
GTP | fragment 87-394 + tRNA + ribosomes + poly(U) | Mycobacterium tuberculosis | |
1.36 | - |
GTP | full length enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the optimal concentration for pppGpp synthesis is approximately equal to the total concentration of nucleotide substrates([ATP] + [GTP]) | Mycobacterium tuberculosis | |
Mn2+ | the optimal concentration for pppGpp synthesis is approximately one-half of the total concentration of nucleotide substrates([ATP] + [GTP]) | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
full-length enzyme, calculated from OD280 | Mycobacterium tuberculosis |
240000 | - |
full-length enzyme, gel filtration | Mycobacterium tuberculosis |
245000 | - |
full-length enzyme, calculated from amino acid sequence | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | Mycobacterium tuberculosis | - |
AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00021 | - |
mol of PPi/s/mol of enzyme, fragment 1-181 | Mycobacterium tuberculosis |
0.027 | - |
mol of PPi/s/mol of enzyme, fragment 1-394 monomer | Mycobacterium tuberculosis |
0.028 | - |
mol of PPi/s/mol of enzyme, full length enzyme | Mycobacterium tuberculosis |
0.03 | - |
mol of PPi/s/mol of enzyme, fragment 1-203 | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | - |
Mycobacterium tuberculosis | AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 82000, gel filtration, calculated from OD280 | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
(p)ppGpp synthetase/hydrolase | - |
Mycobacterium tuberculosis |
RelMtb protein | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
GTP | full length enzyme | Mycobacterium tuberculosis | |
0.4 | - |
GTP | full length enzyme + tRNA | Mycobacterium tuberculosis | |
0.47 | - |
GTP | fragment 87-394 | Mycobacterium tuberculosis | |
0.47 | - |
GTP | fragment 87-394 + tRNA | Mycobacterium tuberculosis | |
0.47 | - |
GTP | fragment 87-394 + tRNA + ribosomes + poly(U) | Mycobacterium tuberculosis | |
0.66 | - |
GTP | fragment 1-394 + tRNA | Mycobacterium tuberculosis | |
0.66 | - |
GTP | fragment 1-394 + tRNA + ribosomes + poly(U) | Mycobacterium tuberculosis | |
0.66 | - |
GTP | fragment 1-394 monomer | Mycobacterium tuberculosis | |
8.4 | - |
GTP | full length enzyme + tRNA + ribosomes + poly(U) | Mycobacterium tuberculosis |