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Literature summary for 2.7.7.1 extracted from

  • Dahmen, W.; Webb, B.; Preiss, J.
    The deamido-diphosphopyridine nucleotide and diphosphopyridine nucleotide pyrophosphorylases of Escherichia coli and yeast (1967), Arch. Biochem. Biophys., 120, 440-450.
    View publication on PubMed
Search for more literature for 2.7.7.1

Activating Compound

Activating Compound Comment Organism Structure
NH4Cl activation, can substitute for KCl Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NAD+ deamide-NAD+ as substrate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 deamido-NAD+ 37°C, pH 7.5 Escherichia coli
0.029
-
 deamido-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
0.06
-
 ATP 37°C, pH 7.5, + nicotinate ribonucleotide Saccharomyces cerevisiae
0.067 0.069 NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
0.08
-
 nicotinate ribonucleotide 37°C, pH 7.5 Escherichia coli
0.13
-
 nicotinate ribonucleotide 37°C, pH 7.5 Saccharomyces cerevisiae
0.147 0.2 nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae
0.147 0.2 nicotinamide ribonucleotide 37°C, pH 7.5 Escherichia coli
0.27
-
 3-acetylpyridine-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
0.37
-
 NAD+ 37°C, pH 7.5 Escherichia coli
0.4
-
 nicotinamide ribonucleotide 37°C, pH 7.5 Saccharomyces cerevisiae
0.5 0.52 ATP 37°C, pH 7.5 Escherichia coli
0.65
-
 diphosphate 37°C, pH 7.5 Saccharomyces cerevisiae
0.74
-
 3-pyridinealdehyde-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
1.1
-
 diphosphate 37°C, pH 7.5 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis Escherichia coli
Mg2+ requirement Escherichia coli
Mg2+ requirement Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Escherichia coli
-
 diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide Escherichia coli B / ATCC 11303
-
 diphosphate + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 B
-
Escherichia coli B / ATCC 11303
-
 B
-
Saccharomyces cerevisiae
-
 brewer's yeast
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0038
-
 NAD+-synthesis Escherichia coli
0.004
-
 NAD+ as substrate Escherichia coli
0.024
-
 deamido-NAD+ as substrate Escherichia coli
0.068
-
 deamido-NAD+-synthesis Escherichia coli
3.3
-
 NAD+-synthesis Saccharomyces cerevisiae
3.85
-
 deamido-NAD+ as substrate Saccharomyces cerevisiae
5
-
 NAD+ as substrate, yeast Saccharomyces cerevisiae
7.3
-
 deamido-NAD+-synthesis Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-acetylpyridine-NAD+ poor substrate Escherichia coli ?
-
 ?
ATP + 3-acetylpyridine-NAD+ reaction at 76% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
 ?
ATP + 3-pyridinealdehyde-NAD+ poor substrate Escherichia coli ?
-
 ?
ATP + 3-pyridinealdehyde-NAD+ reaction at 28% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
 ?
ATP + nicotinamide ribonucleotide
-
 Escherichia coli diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide best substrate Escherichia coli diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide best substrate Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide reverse reaction at 17% the rate of deamido-NAD+-synthesis Escherichia coli diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP Escherichia coli diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Escherichia coli diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide
-
 Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide best substrate Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide reverse reaction at 17% the rate of deamido-NAD+-synthesis Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
ATP + nicotinate ribonucleotide best substrate Escherichia coli diphosphate + nicotinic acid-adenine dinucleotide
-
 r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Escherichia coli diphosphate + nicotinic acid-adenine dinucleotide
-
 r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r
ATP + nicotinate ribonucleotide reaction at 77% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r
deoxy-ATP + nicotinamide ribonucleotide
-
 Escherichia coli ?
-
 ?
deoxy-ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae ?
-
 ?
deoxy-ATP + nicotinate ribonucleotide reaction at 18% the rate of ATP Escherichia coli ?
-
 ?
deoxy-ATP + nicotinate ribonucleotide reaction at 30% the rate of ATP Saccharomyces cerevisiae ?
-
 ?
nicotinate mononucleotide + ATP
-
 Escherichia coli nicotinate adenine dinucleotide + diphosphate
-
 ?
nicotinate mononucleotide + ATP
-
 Saccharomyces cerevisiae nicotinate adenine dinucleotide + diphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min 54% loss of activity Escherichia coli
60
-
 5 min 39% loss of activity Saccharomyces cerevisiae
60
-
 5 min 95% loss of activity Escherichia coli
65
-
 5 min 65% loss of activity Saccharomyces cerevisiae