Literature summary for 2.7.7.1 extracted from

Zhou, T.; Kurnasov, O.; Tomchick, D.R.; Binns, D.D.; Grishin, N.V.; Marquez, V.E.; Osterman, A.L.; Zhang, H.
Structure of human nicotinamide/nicotinic acid mononucleotide adenylyltransferase. Basis for the dual substrate specificity and activation of the oncolytic agent tiazofurin (2002), J. Biol. Chem., 277, 13148-13154.

Search for more literature for 2.7.7.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 with a 6 x His-tag	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, 25 mg/ml protein in 100 mM HEPES, pH 7.2, 0.5 M NaCl, 2 mM dithiothreitol, 1 mM EDTA and 0.03% Brij-35 was incubated with 10 mM NAD+ and then mixed with an equal volume of reservoir solution containing 0.1 mM sodium acetate, pH 4.2-6.4 and 1.6-1.8 mM sodium formate at 20°C for 1-2 weeks, monoclinic space group P21	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + nicotinamide ribonucleotide	Homo sapiens	-	diphosphate + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9HAN9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + nicotinamide ribonucleotide	-	Homo sapiens	diphosphate + NAD+	-	?
ATP + nicotinamide ribonucleotide	i.e. NMN or nicotinamide mononucleotide	Homo sapiens	diphosphate + NAD+	-	r
ATP + nicotinate ribonucleotide	i.e. nicotinate mononucleotide	Homo sapiens	diphosphate + nicotinic acid-adenine dinucleotide	-	r
tiazofurin + ATP	-	Homo sapiens	tiazofurin adenine dinucleotide + diphosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	alpha/beta-topology subunits, X-ray crystallography, barrel-like hexamer	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)