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Literature summary for 2.7.7.1 extracted from

  • Saridakis, V.; Pai, E.F.
    Mutational, structural, and kinetic studies of the ATP-binding site of Methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferase (2003), J. Biol. Chem., 278, 34356-34363.
    View publication on PubMed
Search for more literature for 2.7.7.1

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
H16A slower turnover and higher KM-value for ATP then wild-type Methanothermobacter thermautotrophicus
H19A slower turnover but lower KM-value for ATP then wild-type Methanothermobacter thermautotrophicus
R11A slower turnover and higher KM-value for ATP then wild-type Methanothermobacter thermautotrophicus
R11K slower turnover and higher KM-value for ATP then wild-type Methanothermobacter thermautotrophicus
R136A slower turnover and higher KM-value for ATP then wild-type Methanothermobacter thermautotrophicus
R136K slower turnover and higher KM-value for ATP then wild-type Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 NMN 65°C, pH 7.5, wild-type Methanothermobacter thermautotrophicus
0.08
-
 ATP 65°C, pH 7.5, H19A Methanothermobacter thermautotrophicus
0.09
-
 NMN 65°C, pH 7.5, H19A Methanothermobacter thermautotrophicus
0.11
-
 NMN 65°C, pH 7.5, R136A Methanothermobacter thermautotrophicus
0.13
-
 NMN 65°C, pH 7.5, R11K Methanothermobacter thermautotrophicus
0.14
-
 NMN 65°C, pH 7.5, R136K and R11A Methanothermobacter thermautotrophicus
0.16
-
 ATP 65°C, pH 7.5, wild-type Methanothermobacter thermautotrophicus
0.2
-
 NMN 65°C, pH 7.5, H16A Methanothermobacter thermautotrophicus
0.21
-
 ATP 65°C, pH 7.5, R11K Methanothermobacter thermautotrophicus
0.33
-
 ATP 65°C, pH 7.5, R11A Methanothermobacter thermautotrophicus
0.46
-
 ATP 65°C, pH 7.5, R136K Methanothermobacter thermautotrophicus
1.1
-
 ATP 65°C, pH 7.5, H16A Methanothermobacter thermautotrophicus
1.4
-
 ATP 65°C, pH 7.5, R136A Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Methanothermobacter thermautotrophicus diphosphate + NAD+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000333
-
 ATP 65°C, pH 7.5, H19A Methanothermobacter thermautotrophicus
0.000117
-
 ATP 65°C, pH 7.5, R136A Methanothermobacter thermautotrophicus
0.000133
-
 ATP 65°C, pH 7.5, R11A Methanothermobacter thermautotrophicus
0.000167
-
 ATP 65°C, pH 7.5, H16A Methanothermobacter thermautotrophicus
0.0008
-
 ATP 65°C, pH 7.5, R11K Methanothermobacter thermautotrophicus
0.00222
-
 ATP 65°C, pH 7.5, R136K Methanothermobacter thermautotrophicus
0.00278
-
 ATP 65°C, pH 7.5, wild-type Methanothermobacter thermautotrophicus