Cloned (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
General Stability | Organism |
---|---|
chaotropic anions are strong destabilizers of the enzyme | Saccharolobus solfataricus |
increasing ionic strenght of NaCl and KCl stabilizes the thermal stability of the enzyme | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18600 | - |
x * 18600, SDS-PAGE | Saccharolobus solfataricus |
21500 | - |
x * 21500, SDS-PAGE | Methanocaldococcus jannaschii |
66000 | - |
- |
Saccharolobus solfataricus |
72000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Saccharolobus solfataricus | P57084 | - |
- |
Saccharolobus solfataricus P2 | P57084 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Methanocaldococcus jannaschii | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Saccharolobus solfataricus | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Saccharolobus solfataricus P2 | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Saccharolobus solfataricus | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Saccharolobus solfataricus P2 | diphosphate + nicotinic acid-adenine dinucleotide | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 18600, SDS-PAGE | Saccharolobus solfataricus |
? | x * 21500, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 97 | activity shows a continuous increase up to 97°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for 6 h | Methanocaldococcus jannaschii |
80 | - |
half life: 3 h | Methanocaldococcus jannaschii |
80 | - |
stable at least 2 h | Saccharolobus solfataricus |
85 | - |
half life: 75 min | Saccharolobus solfataricus |
90 | - |
half life: 30 min | Saccharolobus solfataricus |
90 | - |
half life: 1 h | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.8 | - |
Saccharolobus solfataricus |