KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
UTP | pH 7.0, 30°C | Entamoeba histolytica | |
0.23 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C | Entamoeba histolytica | |
0.415 | - |
alpha-D-galactose 1-phosphate | pH 7.0, 30°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Entamoeba histolytica | |
Mn2+ | activation | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-galactose 1-phosphate | Entamoeba histolytica | constitutive enzyme | diphosphate + UDP-galactose | - |
r | |
UTP + alpha-D-galactose 1-phosphate | Entamoeba histolytica K-9 / ATCC 30015 | constitutive enzyme | diphosphate + UDP-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Entamoeba histolytica K-9 / ATCC 30015 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
135 | - |
purified enzyme | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
-20°C, in 20 mM imidazole-HCl buffer, pH 7, 20% v/v glycerol, 30% loss of activity within 6 weeks | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with ATP, ITP, CTP or GTP | Entamoeba histolytica | ? | - |
? | |
additional information | no activity with ATP, ITP, CTP or GTP | Entamoeba histolytica K-9 / ATCC 30015 | ? | - |
? | |
UTP + alpha-D-galactose 1-phosphate | - |
Entamoeba histolytica | diphosphate + UDP-galactose | - |
r | |
UTP + alpha-D-galactose 1-phosphate | constitutive enzyme | Entamoeba histolytica | diphosphate + UDP-galactose | - |
r | |
UTP + alpha-D-galactose 1-phosphate | - |
Entamoeba histolytica K-9 / ATCC 30015 | diphosphate + UDP-galactose | - |
r | |
UTP + alpha-D-galactose 1-phosphate | constitutive enzyme | Entamoeba histolytica K-9 / ATCC 30015 | diphosphate + UDP-galactose | - |
r | |
UTP + alpha-D-glucose 1-phosphate | reaction at 75% the rate of alpha-D-galactose 1-phosphate | Entamoeba histolytica | diphosphate + UDP-glucose | - |
r | |
UTP + alpha-D-glucose 1-phosphate | reaction at 75% the rate of alpha-D-galactose 1-phosphate | Entamoeba histolytica K-9 / ATCC 30015 | diphosphate + UDP-glucose | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Entamoeba histolytica |