General Stability | Organism |
---|---|
glycerol 20% stabilizes | Bifidobacterium bifidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 94% inhibition at 0.2 mM | Bifidobacterium bifidum | |
iodoacetate | no inhibition | Bifidobacterium bifidum | |
additional information | no inhibition by NaF | Bifidobacterium bifidum | |
NEM | no inhibition | Bifidobacterium bifidum | |
PCMB | 42% inhibition at 0.2 mM | Bifidobacterium bifidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
UDP-glucose | pH 8.7, 25°C | Bifidobacterium bifidum | |
0.031 | - |
UDP-galactose | pH 8.7, 25°C | Bifidobacterium bifidum | |
0.14 | 0.16 | alpha-D-glucose 1-phosphate | pH 8.7, 25°C | Bifidobacterium bifidum | |
0.3 | 0.5 | alpha-D-galactose 1-phosphate | pH 8.7, 25°C | Bifidobacterium bifidum | |
6.66 | - |
alpha-D-galactose 1-phosphate | pH 8.7, 25°C | Bifidobacterium bifidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Bifidobacterium bifidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | Bifidobacterium bifidum | involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
constitutive expression | - |
Purification (Comment) | Organism |
---|---|
213fold to homogeneity | Bifidobacterium bifidum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.53 | - |
purified enzyme | Bifidobacterium bifidum |
Storage Stability | Organism |
---|---|
-20°C, in 0.01 M phosphate buffer, pH 7.5: 40% loss of activity within 2 weeks and 60% within a month, with 20% glycerol: 15% loss of activity within 2 months | Bifidobacterium bifidum |
4°C, in 0.01 M potassium phosphate buffer, pH 7.5, inactivation within 2 weeks, 2-mercaptoethanol, DTT and 20% glycerol slightly stabilize | Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-glucose + alpha-D-galactose 1-phosphate | no activity | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + CDP-galactose | - |
? | |
additional information | no activity with CDP-glucose | Bifidobacterium bifidum | ? | - |
? | |
additional information | no activity with ADP-glucose or GDP-glucose | Bifidobacterium bifidum | ? | - |
? | |
TDP-glucose + alpha-D-galactose 1-phosphate | reaction at 60% the rate of UDP-glucose | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + TDP-galactose | - |
r | |
UDP-glucose + alpha-D-galactose 1-phosphate | highly specific for the substrates | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
UDP-glucose + alpha-D-galactose 1-phosphate | involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000-55000, SDS-PAGE | Bifidobacterium bifidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bifidobacterium bifidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
DTT does not prevent heat inactivation | Bifidobacterium bifidum |
40 | - |
10 min, 20% loss of activity | Bifidobacterium bifidum |
50 | - |
above, inactivation | Bifidobacterium bifidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bifidobacterium bifidum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.4 | 9 | about half-maximal activity at pH 4.4 and pH 9.0 | Bifidobacterium bifidum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
and below, very unstable | Bifidobacterium bifidum |
6 | 9 | stable | Bifidobacterium bifidum |