Cloned (Comment) | Organism |
---|---|
expression of wild-type and several PMI-GMP mutants in algA mutant 8853 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
K175E | approx. 9% of wild-type activity, 600fold increase in Km for mannose 1-phosphate | Pseudomonas aeruginosa |
K175Q | approx. 40% of wild-type activity, 3200fold increase in Km for mannose 1-phosphate | Pseudomonas aeruginosa |
K175R | 470fold increase in mannose 1-phosphate Km value | Pseudomonas aeruginosa |
K20Q | enzyme is unable to support alginate synthesis although it shows no significant differences in Vmax and Km as compared to wild-type | Pseudomonas aeruginosa |
R19H | approx. 50% of wild-type activity, 8fold increase in Km for mannose 1-phosphate | Pseudomonas aeruginosa |
R19K | approx. 50% of wild-type activity, 2 and 6fold increase in Km for mannose 1-phosphate and GTP, respectively | Pseudomonas aeruginosa |
R19L | approx. 50% of wild-type activity, 5fold increase in Km for mannose 1-phosphate and GTP, respectively | Pseudomonas aeruginosa |
S12A | approx. 44% of wild-type activity, 2 and 3fold decrease in Km for mannose 1-phosphate and GTP, respectively | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
alpha-D-mannose 1-phosphate | 25°C, cosubstrate GTP | Pseudomonas aeruginosa | |
0.041 | - |
GTP | 25°C, cosubstrate mannose 1-phosphate | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Pseudomonas aeruginosa | - |
GDPmannose + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P07874 | enzyme is encoded by the algA gene | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose | PMI and GMP activities are most probably located in catalytically distinct domains | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | GDPmannose + diphosphate | - |
? | |
GTP + alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | diphosphate + GDP-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PIM-GMP | - |
- |