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Literature summary for 2.7.7.13 extracted from

  • Shinabarger, D.; Berry, A.; May, T.B.; Rothmel, R.; Fialho, A.; Chakrabarty, A.M.
    Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa (1991), J. Biol. Chem., 266, 2080-2088.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by dithiothreitol up to 5 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0142
-
GDPmannose pH 7.0, 25°C Pseudomonas aeruginosa
0.0205
-
alpha-D-mannose 1-phosphate pH 8.0, 25°C Pseudomonas aeruginosa
0.0295
-
GTP pH 8.0, 25°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ required for activity Pseudomonas aeruginosa
Mn2+ Mg2+ or Mn2+ required for activity Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
gel filtration, phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is a bifunctional enzyme catalyzing both the phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, reaction Pseudomonas aeruginosa
56000
-
1 * 56000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Pseudomonas aeruginosa enzyme of alginate biosynthetic pathway GDPmannose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P07874 phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is a bifunctional enzyme catalyzing both phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, reactions
-

Purification (Commentary)

Purification (Comment) Organism
Biogel, Q-Sepharose, Sephacryl-200 Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose enzyme has both phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate no activity with GDP-D-glucose, ADP-D-mannose and UDP-D-mannose Pseudomonas aeruginosa GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate enzyme of alginate biosynthetic pathway Pseudomonas aeruginosa GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate
-
Pseudomonas aeruginosa diphosphate + GDP-mannose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PIM-GMP
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas aeruginosa