Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-glucose | 2.5 mM, 50% inhibition | Mycolicibacterium smegmatis | |
glucose 1-phosphate | 10 mM, 50% inhibition | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
GTP | pH 7.5, 37°C, cosubstrate mannose 1-phosphate | Mycolicibacterium smegmatis | |
0.14 | - |
GDPmannose | pH 7.5, 37°C, cosubstrate diphosphate | Mycolicibacterium smegmatis | |
0.168 | - |
alpha-D-mannose 1-phosphate | pH 7.5, 37°C, cosubstrate GTP | Mycolicibacterium smegmatis | |
0.356 | - |
diphosphate | pH 7.5, 37°C, cosubstrate GDPmannose | Mycolicibacterium smegmatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mycolicibacterium smegmatis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required for activity, second best activator, maximal activation at 5 mM | Mycolicibacterium smegmatis | |
Mg2+ | divalent cation required for activity, best metal activator Mg2+, maximal activation at 5-10 mM | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Mycolicibacterium smegmatis |
70000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Mycolicibacterium smegmatis | - |
GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-52, ammonium sulfate, propyl-agarose, methyl-agrose, Sephadex G-200, hydroxyapatite | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.99 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
0°C, 1 week, 50% loss of activity | Mycolicibacterium smegmatis |
frozen, several months, little loss of activity | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | - |
Mycolicibacterium smegmatis | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | no activity with glucose 1-phosphate, UTP, ATP and CTP | Mycolicibacterium smegmatis | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Mycolicibacterium smegmatis | diphosphate + GDP-mannose | - |
? | |
ITP + alpha-D-mannose 1-phosphate | 15-20% of activity with GTP | Mycolicibacterium smegmatis | IDPmannose + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Mycolicibacterium smegmatis |