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Literature summary for 2.7.7.13 extracted from

  • Ning, B.; Elbein, A.D.
    Purification and properties of mycobacterial GDP-mannose pyrophosphorylase (1999), Arch. Biochem. Biophys., 362, 339-345.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GDP-glucose 2.5 mM, 50% inhibition Mycolicibacterium smegmatis
glucose 1-phosphate 10 mM, 50% inhibition Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.113
-
GTP pH 7.5, 37°C, cosubstrate mannose 1-phosphate Mycolicibacterium smegmatis
0.14
-
GDPmannose pH 7.5, 37°C, cosubstrate diphosphate Mycolicibacterium smegmatis
0.168
-
alpha-D-mannose 1-phosphate pH 7.5, 37°C, cosubstrate GTP Mycolicibacterium smegmatis
0.356
-
diphosphate pH 7.5, 37°C, cosubstrate GDPmannose Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mycolicibacterium smegmatis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required for activity, second best activator, maximal activation at 5 mM Mycolicibacterium smegmatis
Mg2+ divalent cation required for activity, best metal activator Mg2+, maximal activation at 5-10 mM Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Mycolicibacterium smegmatis
70000
-
gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Mycolicibacterium smegmatis
-
GDPmannose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-52, ammonium sulfate, propyl-agarose, methyl-agrose, Sephadex G-200, hydroxyapatite Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.99
-
-
Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
0°C, 1 week, 50% loss of activity Mycolicibacterium smegmatis
frozen, several months, little loss of activity Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate
-
Mycolicibacterium smegmatis GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate no activity with glucose 1-phosphate, UTP, ATP and CTP Mycolicibacterium smegmatis GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate
-
Mycolicibacterium smegmatis diphosphate + GDP-mannose
-
?
ITP + alpha-D-mannose 1-phosphate 15-20% of activity with GTP Mycolicibacterium smegmatis IDPmannose + diphosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Mycolicibacterium smegmatis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
Mycolicibacterium smegmatis